Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody

GAPDH Antibody (C-term R248)

Cat. Num. AAA28693

• Gene Names: GAPDH; G3PD; GAPD; HEL-S-162eP
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GAPDH; Polyclonal Antibody; GAPDH Antibody (C-term R248); Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH; 2699-; GAPD; anti-GAPDH antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 233-259
• Sequence Length: 293

• Applicable Applications for anti-GAPDH antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: WB~~1:1000; IHC-p~~1:50~100; IF~~1:10~50; FC~~1:10~50
• Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D- glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
• Cellular Location: Cytoplasm, cytosol. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261}
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM (Flow Cytometry)

(Flow cytometric analysis of HepG2 cells using GAPDH Antibody (C-term R248)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of GAPDH Antibody (C-term R248) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (C-term R248). AAA28693 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

Related Product Information for anti-GAPDH antibody

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Product Categories/Family for anti-GAPDH antibody

Signal Transduction; Neuroscience; Microbiology; Metabolism; Cardiovascular; Cancer; Phospho Antibodies

References

Azam,S., J. Biol. Chem. 283 (45), 30632-30641 (2008)
Lu,J., Biosci. Biotechnol. Biochem. 72 (9), 2432-2435 (2008)
Zhou,Y., Mol. Cancer Res. 6 (8), 1375-1384 (2008)

NCBI and Uniprot Product Information

• NCBI GI #: 378404908
• NCBI GeneID: 2597
• NCBI Accession #: NP_001243728.1; NP_002037.2
• NCBI GenBank Nucleotide #: NM_001256799.2
• UniProt Accession #: P04406; P00354; Q53X65; E7EUT4
• Molecular Weight: 36053
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD; HEL-S-162eP
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH
• UniProt Entry Name: G3P_HUMAN