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8299 results for "Phosphorylation" - showing 900-950


product-image-AAA111779_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells, untreated or treated with serum(15%), using p70 S6 Kinase (Ab-424) antibody (Line 1 and 2) and p70 S6 Kinase (Phospho-Ser424) antibody (Line 3 and 4).)

p70 S6 Kinase, Antibody (Cat# AAA111779)

Full Name
p70 S6 Kinase (Phospho-Ser424) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA144411_SDS_PAGE15.jpg SDS-PAGE

Nucleoporin, Recombinant Protein (Cat# AAA144411)

Full Name
Recombinant Nucleoporin 214kDa (NUP214)
Reactivity
Mus musculus (Mouse)
Applications
Western Blot
Purity
> 97%
Pricing
product-image-AAA172564_WB13.jpg WB (Western Blot) (Western Blot analysis of 3T3 cells with Casein Kinase I? Polyclonal Antibody.)

Casein Kinase Ialpha, Polyclonal Antibody (Cat# AAA172564)

Full Name
Casein Kinase Ialpha Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
product-image-AAA134189_SDS_PAGE11.jpg SDS-PAGE

Transglutaminase 2, Tissue (TGM2), Recombinant Protein (Cat# AAA134189)

Full Name
Recombinant Transglutaminase 2, Tissue (TGM2)
Gene Names
TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
Applications
Western Blot
Purity
> 80%
Pricing
product-image-AAA288666_WB15.jpg WB (Western Blot) (MYOG Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MYOG antibody detected the MYOG protein (arrow).)

MYOG, Polyclonal Antibody (Cat# AAA288666)

Full Name
MYOG Antibody (Center)
Gene Names
MYOG; MYF4; myf-4; bHLHc3
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
product-image-AAA321411_IHC13.jpg IHC (Immunohiostchemistry) (AAA321411 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

TrkA, Polyclonal Antibody (Cat# AAA321411)

Full Name
Phospho-TrkA (Tyr490) Antibody
Gene Names
NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321398_IHC13.jpg IHC (Immunohiostchemistry) (AAA321398 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Survivin, Polyclonal Antibody (Cat# AAA321398)

Full Name
Phospho-Survivin (Thr34) Antibody
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA19845_FCM15.jpg FCM (Flow Cytometry) (Figure 15. Flow Cytometry analysis of U251 cells using anti-ARS2/SRRT antibody (AAA19845).Overlay histogram showing U251 cells stained with AAA19845 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARS2/SRRT Antibody (AAA19845, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

ARS2/SRRT, Polyclonal Antibody (Cat# AAA19845)

Full Name
Anti-ARS2/SRRT Antibody Picoband
Gene Names
SRRT; ARS2; ASR2; serrate
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA290456_ICC13.jpg ICC (Immunocytochemistry) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)

Histone H2A.X, Monoclonal Antibody (Cat# AAA290456)

Full Name
Phospho-Histone H2A.X (Ser139) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Pricing
product-image-AAA306901_WB13.jpg WB (Western Blot) (Western Blot analysis of various cells using EF-2 Polyclonal Antibody)

EF-2, Polyclonal Antibody (Cat# AAA306901)

Full Name
EF-2 Polyclonal Antibody
Gene Names
EEF2; EF2; EF-2; EEF-2; SCA26
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA187466_SC15.jpg Standard Curve (Sample)

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), ELISA Kit (Cat# AAA187466)

Full Name
Mouse Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) ELISA Kit
Gene Names
DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
Reactivity
Mouse
Pricing
product-image-AAA236780_WB10.jpg WB (Western Blot) (Western Blot analysis of HELA HEPG2 using c-Src Polyclonal Antibody)

SRC, Polyclonal Antibody (Cat# AAA236780)

Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA303169_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using S6 Ribosomal Protein(Phospho-Ser235/236) Antibody.)

S6 Ribosomal Protein, Polyclonal Antibody (Cat# AAA303169)

Full Name
S6 Ribosomal Protein (Phospho-Ser235/236) Antibody
Gene Names
RPS6; S6
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA37411_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-GSK3B-Y216 antibody.)

GSK3B-Y216, Antibody (Cat# AAA37411)

Full Name
Phospho-GSK3B-Y216 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
product-image-AAA316169_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) Antibody. The lane on the right is blocked with the phospho peptide.)

CDK1/CDC2, Polyclonal Antibody (Cat# AAA316169)

Full Name
CDK1/CDC2 (Phospho-Thr14) Antibody
Gene Names
CDK2; CDKN2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA236744_WB13.jpg WB (Western Blot) (Western Blot analysis of AD293 22RV1 HELA cells using Neu Polyclonal Antibody)

ERBB2, Polyclonal Antibody (Cat# AAA236744)

Full Name
ERBB2 Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA110968_WB13.jpg WB (Western Blot) (UV-+ Western blot analysis of extracts from NIH/3T3 cells using c-Jun (phospho-Ser63) antibody)

c-Jun, Antibody (Cat# AAA110968)

Full Name
c-Jun (Phospho-Ser63) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA316330_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.)

CD5, Polyclonal Antibody (Cat# AAA316330)

Full Name
CD5 (Phospho-Tyr453) Antibody
Gene Names
CD5; T1; LEU1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA316332_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with etoposide 25uM 24h, using CDC25B (Phospho-Ser353) Antibody. The lane on the right is blocked with the phospho peptide.)

CDC25B, Polyclonal Antibody (Cat# AAA316332)

Full Name
CDC25B (Phospho-Ser353) Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA243090_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser807) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

RB1, Polyclonal Antibody (Cat# AAA243090)

Full Name
Phospho-RB1 (Ser807) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA111087_IHC15.jpg IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BRCA1 (phospho-Ser1524) antibody.)

BRCA1, Antibody (Cat# AAA111087)

Full Name
BRCA1 (Phospho-Ser1524) Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA316153_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with UV 15', using Integrin beta1 (Phospho-Thr788) Antibody. The lane on the right is blocked with the phospho peptide.)

Integrin beta1, Polyclonal Antibody (Cat# AAA316153)

Full Name
Integrin beta1 (Phospho-Thr788) Antibody
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA111707_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of HuvEC cells, using Telomerase (Phospho-Ser227) antibody. Telomerase (Phospho-Ser227) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Telomerase, Antibody (Cat# AAA111707)

Full Name
Telomerase (Phospho-Ser227) Antibody
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
Immunofluorescence
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA307228_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody.)

c-Jun, Polyclonal Antibody (Cat# AAA307228)

Full Name
c-Jun (Phospho-Ser73) Antibody
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA201106_WB11.jpg WB (Western Blot) (WB Suggested Anti-Epha4 AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Pancreas)

Epha4, Polyclonal Antibody (Cat# AAA201106)

Full Name
Epha4 antibody - C-terminal region
Gene Names
Epha4; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
product-image-AAA111175_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase (Ab-204) antibody and p44/42 MAP Kinase (phospho-Tyr204) antibody.)

p44/42 MAP Kinase, Antibody (Cat# AAA111175)

Full Name
p44/42 MAP Kinase (Phospho-Tyr204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA243271_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Phospho-Tyr1214) Antibody.)

KDR, Polyclonal Antibody (Cat# AAA243271)

Full Name
Phospho-KDR (Tyr1214) Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA321505_WB13.jpg WB (Western Blot) (Western blot analysis of Phospho-PDGFR beta (Tyr1021) Antibody expression in Rat kidney and mouse kidney tissues lysates.)

PDGFR beta, Polyclonal Antibody (Cat# AAA321505)

Full Name
Phospho-PDGFR beta (Tyr1021) Antibody
Gene Names
PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA302321_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left)or the same antibody preincubated with blocking peptide (right).)

p47 phox, Polyclonal Antibody (Cat# AAA302321)

Full Name
p47 phox (Phospho-Ser345) Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA243109_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using APP (Phospho-Thr668) Antibody.)

APP, Polyclonal Antibody (Cat# AAA243109)

Full Name
Phospho-APP (Thr668) Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA144390_SDS_PAGE11.jpg SDS-PAGE

Latent Transforming Growth Factor Beta Binding Protein 1, Recombinant Protein (Cat# AAA144390)

Full Name
Recombinant Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1)
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 95%
Pricing
product-image-AAA144139_SDS_PAGE15.jpg SDS-PAGE

Forkhead Box Protein P1, Recombinant Protein (Cat# AAA144139)

Full Name
Recombinant Forkhead Box Protein P1 (FOXP1)
Gene Names
Foxp1; AI461938; AW494214; 3110052D19Rik; 4932443N09Rik
Applications
Western Blot
Purity
> 95%
Pricing
product-image-AAA150146_BIOACTIVITY11.jpg Bioactivity (Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45a) is a member of the GADD45 family. It may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Besides, Proliferating Cell Nuclear Antigen (PCNA) has been identified as an interactor of GADD45a, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GADD45a and recombinant rat PCNA. Briefly, GADD45a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to PCNA-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-GADD45a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GADD45a and PCNA was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of GADD45a with PCNA.)

Growth Arrest And DNA Damage Inducible Protein Alpha, Active Protein (Cat# AAA150146)

Full Name
Active Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)
Gene Names
Gadd45a; Ddit1; Gadd45
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
product-image-AAA321284_IF11.jpg IF (Immunofluorescence) (AAA321284 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

AKT1/2/3, Polyclonal Antibody (Cat# AAA321284)

Full Name
Phospho-AKT1/2/3(Tyr315/316/312) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321445_IF10.jpg IF (Immunofluorescence) (AAA321445 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Abl, Polyclonal Antibody (Cat# AAA321445)

Full Name
Phospho-Abl (Tyr393/412) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA309145_WB15.jpg WB (Western Blot) (Western blot analysis nrf2(Phospho-S40) using HT-29 whole cell lysates)

nrf2, Polyclonal Antibody (Cat# AAA309145)

Full Name
nrf2(Phospho-S40) Antibody
Gene Names
NFE2L2; NRF2; HEBP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification using immunogen.
Pricing
product-image-AAA172558_IF11.jpg IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-ERK 1/2 (Tyr204) Polyclonal Antibody at dilution of 1:200)

ERK 1/2, Polyclonal Antibody (Cat# AAA172558)

Full Name
Phospho-ERK 1/2 (Y204) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
product-image-AAA318404_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from Jurkat cells and HepG2 cells, using Tryptophan Hydroxylase (Phospho-Ser260) Antibody.)

Tryptophan Hydroxylase, Polyclonal Antibody (Cat# AAA318404)

Full Name
Tryptophan Hydroxylase (Phospho-Ser260) Antibody
Gene Names
TPH1; TPRH; TRPH
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA36739_WB15.jpg WB (Western Blot) (Western blot analysis of extracts of Raji cells, using Phospho-BRAF-S445 antibody at 1:2000 dilution. Raji cells were treated by PMA/TPA (200nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)

BRAF-S445, Antibody (Cat# AAA36739)

Full Name
Phospho-BRAF-S445
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Pricing
product-image-AAA316563_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from COS7 cells and A549 cells, using PKC delta (Phospho-Ser645) Antibody. The lane on the right is blocked with the phospho peptide.)

PKC delta, Polyclonal Antibody (Cat# AAA316563)

Full Name
PKC delta (Phospho-Ser645) Antibody
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA318308_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody.)

eNOS, Polyclonal Antibody (Cat# AAA318308)

Full Name
eNOS (Phospho-Ser615) Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA39260_WB11.gif WB (Western Blot) (Western blot analysis of DYRK1A expression in K562 cell lysate with DYRK1A antibody at (A) 1 and (B) 2 μg/ml.)

DYRK1A, Polyclonal Antibody (Cat# AAA39260)

Full Name
DYRK1A Antibody
Gene Names
DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Purity
DYRK1A Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA307250_IF13.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using S6 Ribosomal Protein(Phospho-Ser235) Antibody.)

S6 Ribosomal Protein, Polyclonal Antibody (Cat# AAA307250)

Full Name
S6 Ribosomal Protein (Phospho-Ser235) Antibody
Gene Names
RPS6; S6
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
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product-image-AAA303007_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using beta-catenin (phospho-Tyr333) Antibody.)

beta-catenin, Polyclonal Antibody (Cat# AAA303007)

Full Name
beta-catenin (phospho-Tyr333) Antibody
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
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product-image-AAA243225_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody (left)or the same antibody preincubated with blocking peptide (right).)

MAPKAPK2, Polyclonal Antibody (Cat# AAA243225)

Full Name
Phospho-MAPKAPK2 (Ser272) Antibody
Gene Names
MAPKAPK2; MK2; MK-2; MAPKAP-K2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
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product-image-AAA111783_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with IFN-alpha (1000U/ml, 18 hours), using eIF2alpha (Ab-51) antibody (Line 1 and 2) and eIF2alpha (phospho-Ser51) antibody (Line 3 and 4).)

eIF2alpha, Antibody (Cat# AAA111783)

Full Name
eIF2alpha (Phospho-Ser51) Antibody
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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product-image-AAA316302_WB11.jpg WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKC delta (Phospho-Tyr52) Antibody. The lane on the right is blocked with the phospho peptide.)

PKC delta, Polyclonal Antibody (Cat# AAA316302)

Full Name
PKC delta (Phospho-Tyr52) Antibody
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA303836_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using LIMK2(Phospho-Thr505) Antibody.)

LIMK2, Polyclonal Antibody (Cat# AAA303836)

Full Name
LIMK2 (Phospho-Thr505) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA112116_IHC13.jpg IHC (Immunohiostchemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYPT1 (Phospho-Thr696) antibody. Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) antibody.)

MYPT1, Antibody (Cat# AAA112116)

Full Name
MYPT1 (Phospho-Thr696) Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109607_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) antibody.)

DARPP-32, Antibody (Cat# AAA109607)

Full Name
DARPP-32 (Phospho-Thr34) Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

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