Search results for "Recombinant Proteins"

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using INTS6 antibody. Blue: DAPI for nuclear staining.)

INTS6, Polyclonal Antibody (Cat# AAA28131)

Full Name

INTS6 Polyclonal Antibody

Gene Names

INTS6; HDB; INT6; DBI-1; DDX26; DICE1; DDX26A; Notchl2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens).)

C9orf72, Polyclonal Antibody (Cat# AAA28290)

Full Name

C9orf72 Polyclonal Antibody

Gene Names

C9orf72; ALSFTD; DENND9; FTDALS; DENNL72; FTDALS1

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M07), clone 1B7.Lane 1: SMAD3 transfected lysate (48 KDa).Lane 2: Non-transfected lysate.)

SMAD3, Monoclonal Antibody (Cat# AAA26647)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (PE)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Western Blot

Purity

Purified

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-D2AP antibody (M01756). Overlay histogram showing K562 cells stained with M01756 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-D2AP Antibody (M01756, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CD2AP, Monoclonal Antibody (Cat# AAA19185)

Full Name

Anti-CD2AP Antibody (monoclonal, 5F8)

Gene Names

CD2AP; CMS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen Affinity Purified

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Hela cells using anti-PDE4D antibody (AAA19143).Overlay histogram showing Hela cells stained with AAA19143 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4D Antibody (AAA19143,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PDE4D, Polyclonal Antibody (Cat# AAA19143)

Full Name

Anti-PDE4D Picoband antibody

Gene Names

PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Western Blot

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of THP-1 cells using anti-RPL11 antibody (AAA19783).Overlay histogram showing THP-1 cells stained with AAA19783 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL11 Antibody (AAA19783, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of RPL11 using anti-RPL11 antibody (AAA19783).RPL11 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL11 Antibody (AAA19783) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL11 Antibody (AAA19783) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL11 Antibody (AAA19783) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL11 antigen affinity purified polyclonal antibody (#AAA19783) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPL11 at approximately 18 kDa. The expected band size for RPL11 is at 18 kDa.)

RPL11, Polyclonal Antibody (Cat# AAA19783)

Full Name

Anti-RPL11 Antibody Picoband

Gene Names

RPL11; L11; DBA7; GIG34

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-EXOSC3 antibody (AAA19507).Overlay histogram showing PC-3 cells stained with AAA19507 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EXOSC3 Antibody (AAA19507, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

EXOSC3, Polyclonal Antibody (Cat# AAA19507)

Full Name

Anti-EXOSC3 Antibody Picoband

Gene Names

EXOSC3; p10; PCH1B; RRP40; Rrp40p; CGI-102; hRrp-40; bA3J10.7

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

< >

MSK2/RSK-B/RPS6KA4, Polyclonal Antibody (Cat# AAA19553)

Full Name

Anti-MSK2/RSK-B/RPS6KA4 Antibody Picoband

Gene Names

RPS6KA4; MSK2; RSK-B

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)

BANF1, Monoclonal Antibody (Cat# AAA26310)

Full Name

BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (FITC)

Gene Names

BANF1; BAF; NGPS; BCRP1; D14S1460

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody at dilution of 1:100 (40x lens).)

AK1, Polyclonal Antibody (Cat# AAA28266)

Full Name

AK1 Polyclonal Antibody

Gene Names

AK1; HTL-S-58j

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA24716)

Full Name

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (Biotin)

Gene Names

AFP; AFPD; FETA; HPAFP

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPL19 on HeLa cell. [antibody concentration 10ug/ml])

RPL19, Monoclonal Antibody (Cat# AAA25523)

Full Name

RPL19 (60S Ribosomal Protein L19, DKFZp779D216, FLJ27452, HGNC:10312, MGC71997) (HRP)

Gene Names

RPL19; L19

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPL19 on HeLa cell. [antibody concentration 10ug/ml])

RPL19, Monoclonal Antibody (Cat# AAA25819)

Full Name

RPL19 (60S Ribosomal Protein L19, DKFZp779D216, FLJ27452, HGNC:10312, MGC71997) (PE)

Gene Names

RPL19; L19

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)

STIP1, Monoclonal Antibody (Cat# AAA26183)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)

CSTF3, Monoclonal Antibody (Cat# AAA24180)

Full Name

CSTF3 (Cleavage Stimulation Factor Subunit 3, CF-1 77kD Subunit, Cleavage Stimulation Factor 77kD Subunit, CSTF 77kD Subunit, CstF-77) (AP)

Gene Names

CSTF3; CSTF-77

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Figure 6. IHC analysis of GLO1 using anti-GLO1 antibody (AAA19155).GLO1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GLO1 Antibody (AAA19155) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GLO1/Glyoxalase I, Polyclonal Antibody (Cat# AAA19155)

Full Name

Anti-GLO1/Glyoxalase I Picoband antibody

Gene Names

GLO1; GLYI; GLOD1; HEL-S-74

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U-87 cells using anti-MVP antibody (AAA19137).Overlay histogram showing U-87 cells stained with AAA19137 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (AAA19137,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MVP, Polyclonal Antibody (Cat# AAA19137)

Full Name

Anti-MVP Picoband antibody

Gene Names

MVP; LRP; VAULT1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Western Blot

Pricing

< >

TAF4, Polyclonal Antibody (Cat# AAA19307)

Full Name

Anti-TAF4 Antibody

Gene Names

TAF4; TAF2C; TAF4A; TAF2C1; TAFII130; TAFII135

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-C2orf43/LDAH antibody (AAA19990).Overlay histogram showing JK cells stained with AAA19990 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C2orf43/LDAH Antibody (AAA19990, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of C2orf43/LDAH using anti-C2orf43/LDAH antibody (AAA19990).C2orf43/LDAH was detected in a paraffin-embedded section of rat alcoholic liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C2orf43/LDAH Antibody (AAA19990) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C2orf43/LDAH Antibody (AAA19990) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C2orf43/LDAH Antibody (AAA19990) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C2orf43/LDAH Antibody (AAA19990) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: rat RH-35 whole cell lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse kidney tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody (#AAA19990) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: monkey COS-7 whole cell lysates,Lane 6: human SK-N-SH whole cell lysates,Lane 7: human SH-SY5Y whole cell lysates,Lane 8: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody (#AAA19990) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C2orf43/LDAH at approximately 37 kDa. The expected band size for C2orf43/LDAH is at 37 kDa.)

LDAH, Polyclonal Antibody (Cat# AAA19990)

Full Name

Anti-LDAH Antibody Picoband

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-STON1 antibody (AAA19981).Overlay histogram showing 293T cells stained with AAA19981 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STON1 Antibody (AAA19981, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of STON1 using anti-STON1 antibody (AAA19981).STON1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STON1 Antibody (AAA19981) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STON1 Antibody (AAA19981) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STON1 Antibody (AAA19981) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STON1 Antibody (AAA19981) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STON1 antigen affinity purified polyclonal antibody (#AAA19981) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STON1 at approximately 83 kDa. The expected band size for STON1 is at 83 kDa.)

STON1, Polyclonal Antibody (Cat# AAA19981)

Full Name

Anti-STON1 Antibody Picoband

Gene Names

STON1-GTF2A1L; SALF

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HeLa cells using anti-Nucleostemin/GNL3 antibody (AAA19482).Overlay histogram showing HeLa cells stained with AAA19482 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nucleostemin/GNL3 Antibody (AAA19482, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Nucleostemin/GNL3, Polyclonal Antibody (Cat# AAA19482)

Full Name

Anti-Nucleostemin/GNL3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

EYFP, Polyclonal Antibody (Cat# AAA29667)

Full Name

EYFP Rabbit Polyclonal Antibody

Applications

Western Blot

Purity

Affinity purification using immunogen.

Pricing

IL-1RA, ELISA Kit (Cat# AAA17868)

Full Name

Mouse IL-1RA ELISA Kit

Gene Names

Il1rn; IL-1ra; F630041P17Rik

Reactivity

Mouse

Applications

Sandwich ELISA

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using SMAD9 antibody. Blue: DAPI for nuclear staining.)

SMAD9, Polyclonal Antibody (Cat# AAA28113)

Full Name

SMAD9

Gene Names

SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using RPL13 antibody. Blue: DAPI for nuclear staining.)

RPL13, Polyclonal Antibody (Cat# AAA28110)

Full Name

RPL13 Polyclonal Antibody

Gene Names

RPL13; L13; BBC1; D16S44E; D16S444E

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

< >

TP53BP1, Polyclonal Antibody (Cat# AAA28106)

Full Name

TP53BP1 Polyclonal Antibody

Gene Names

TP53BP1; p202; 53BP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M07), clone 1B7.Lane 1: SMAD3 transfected lysate (48 KDa).Lane 2: Non-transfected lysate.)

SMAD3, Monoclonal Antibody (Cat# AAA26382)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (FITC)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Western Blot

Purity

Purified

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

HSPB1, Monoclonal Antibody (Cat# AAA25721)

Full Name

HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) (PE)

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Figure 6. IHC analysis of S100A10 using anti-S100A10 antibody (AAA19162).S100A10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-S100A10 Antibody (AAA19162) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

S100A10, Polyclonal Antibody (Cat# AAA19162)

Full Name

Anti-S100A10 Picoband antibody

Gene Names

S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (AAA19166).Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Thrombopoietin Antibody (AAA19166) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Thrombopoietin, Polyclonal Antibody (Cat# AAA19166)

Full Name

Anti-Thrombopoietin Picoband Antibody

Gene Names

Thpo; Ml; Tpo; Mgdf; Mpllg

Reactivity

Mouse, Rat
No cross reactivity with other proteins

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A549 cells using anti-Transketolase/TKT antibody (AAA19368).Overlay histogram showing A549 cells stained with AAA19368 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Transketolase/TKT Antibody (AAA19368, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Transketolase/TKT, Monoclonal Antibody (Cat# AAA19368)

Full Name

Anti-Transketolase/TKT Antibody (monoclonal, 2I3)

Gene Names

TKT; TK; TKT1; HEL107

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

TIMM8A/DDP, Polyclonal Antibody (Cat# AAA19317)

Full Name

Anti-TIMM8A/DDP Antibody

Gene Names

TIMM8A; DDP; MTS; DDP1; DFN1; TIM8

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-Geminin/GMNN antibody (AAA19563).Overlay histogram showing A431 cells stained with AAA19563 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Geminin/GMNN Antibody (AAA19563, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Geminin/GMNN, Polyclonal Antibody (Cat# AAA19563)

Full Name

Anti-Geminin/GMNN Antibody Picoband

Gene Names

GMNN; Gem

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HEL cells using anti-PSMB4 antibody (AAA19537).Overlay histogram showing HEL cells stained with AAA19537 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB4 Antibody (AAA19537, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PSMB4, Polyclonal Antibody (Cat# AAA19537)

Full Name

Anti-PSMB4 Antibody Picoband

Gene Names

PSMB4; HN3; HsN3; PROS26; PROS-26

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

PCNA, Monoclonal Antibody (Cat# AAA19657)

Full Name

Anti-PCNA Antibody Picoband (monoclonal, 2G2)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

NASP, Polyclonal Antibody (Cat# AAA28154)

Full Name

NASP

Gene Names

NASP; FLB7527; PRO1999

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

RPS23, Monoclonal Antibody (Cat# AAA24343)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) (AP)

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (34.21kD).)

SMAD5, Monoclonal Antibody (Cat# AAA24364)

Full Name

SMAD5 (Mothers Against Decapentaplegic Homolog 5, Mothers Against DPP Homolog 5, MAD Homolog 5, Mad5, SMAD Family Member 5, SMAD 5, Smad5, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323) (AP)

Gene Names

SMAD5; DWFC; JV5-1; MADH5

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

PLK4, Polyclonal Antibody (Cat# AAA28258)

Full Name

PLK4 Polyclonal Antibody

Gene Names

PLK4; SAK; STK18; MCCRP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TSC22D3 antibody. Blue: DAPI for nuclear staining.)

TSC22D3, Polyclonal Antibody (Cat# AAA28179)

Full Name

TSC22D3 Polyclonal Antibody

Gene Names

TSC22D3; DIP; GILZ; hDIP; DSIPI; TSC-22R

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using p70 S6 Kinase 1 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

p70 S6 Kinase 1, Polyclonal Antibody (Cat# AAA28344)

Full Name

p70 S6 Kinase 1 Rabbit pAb

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Anti-GP73 rabbit polyclonal antibody at 1:500 dilution Lane A: GOLM1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size: kDa)

GOLPH2/GOLM1, Polyclonal Antibody (Cat# AAA27751)

Full Name

Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal

Gene Names

GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein A & Antigen Affinity

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED18, Polyclonal Antibody (Cat# AAA19179)

Full Name

Anti-MED18 Picoband Antibody

Gene Names

MED18; SRB5; p28b

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified

Pricing

< >

<< Previous Next >>

Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Emerin, Monoclonal Antibody (Cat# AAA23898)

Full Name

Emerin (Papillary Thyroid Carcinoma and EDMD Marker)

Gene Names

EMD; STA; EDMD; LEMD5

Reactivity

Human. Others not known.

Applications

Immunohistochemistry

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9/Rad9a antibody (AAA19288).Overlay histogram showing HEPA1-6 cells stained with AAA19288 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9/Rad9a Antibody (AAA19288,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Rad9/Rad9a, Polyclonal Antibody (Cat# AAA19288)

Full Name

Anti-Rad9/Rad9a Antibody

Gene Names

Rad9a; Rad9

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Caco-2 cells using anti-BCCIP antibody (AAA19854).Overlay histogram showing Caco-2 cells stained with AAA19854 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCCIP Antibody (AAA19854, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of BCCIP using anti-BCCIP antibody (AAA19854).BCCIP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCCIP antigen affinity purified polyclonal antibody (#AAA19854) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCCIP at approximately 45 kDa. The expected band size for BCCIP is at 36 kDa.)