Search results for "Recombinant Proteins"

SAA1, ELISA Kit (Cat# AAA27871)

Full Name

Human SAA1 ELISA Kit

Gene Names

SAA1; SAA; PIG4; SAA2; TP53I4

Reactivity

Human

Pricing

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WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in Hs 181.Tes.)

SMAD3, Monoclonal Antibody (Cat# AAA26429)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (HRP)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of SCG10/STMN2 using anti- SCG10/STMN2 antibody (AAA19299).SCG10/STMN2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SCG10/STMN2 Antibody (AAA19299) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

SCG10/STMN2, Polyclonal Antibody (Cat# AAA19299)

Full Name

Anti-SCG10/STMN2 Antibody

Gene Names

STMN2; SCG10; SCGN10

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-APLP2 antibody (AAA19746).Overlay histogram showing HepG2 cells stained with AAA19746 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APLP2 Antibody (AAA19746, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of APLP2 using anti-APLP2 antibody (AAA19746).APLP2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (#AAA19746) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa.)

APLP2, Polyclonal Antibody (Cat# AAA19746)

Full Name

Anti-APLP2 Antibody Picoband

Gene Names

APLP2; APPH; APPL2; CDEBP; APLP-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Filamin B/FLNB, Polyclonal Antibody (Cat# AAA19463)

Full Name

Anti-Filamin B/FLNB Antibody Picoband

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody at dilution of 1:100 (40x lens).)

ACTR2, Polyclonal Antibody (Cat# AAA28152)

Full Name

ACTR2 Polyclonal Antibody

Gene Names

ACTR2; ARP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain using Cytokeratin 14 (KRT14) Rabbit mAb (AAA28471) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

Cytokeratin 14 (KRT14), Monoclonal Antibody (Cat# AAA28471)

Full Name

Cytokeratin 14 (KRT14) Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-ASC/TMS1/PYCARD antibody (AAA19733).Overlay histogram showing THP-1 cells stained with AAA19733 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (AAA19733).ASC/TMS1/PYCARD was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HL-60 whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (#AAA19733) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.)

ASC/TMS1/PYCARD, Polyclonal Antibody (Cat# AAA19733)

Full Name

Anti-ASC/TMS1/PYCARD Antibody Picoband

Gene Names

PYCARD; ASC; TMS; TMS1; CARD5; TMS-1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-MT-CO2 antibody (AAA19493).Overlay histogram showing U87 cells stained with AAA19493 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT-CO2 Antibody (AAA19493, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MT-CO2, Polyclonal Antibody (Cat# AAA19493)

Full Name

Anti-MT-CO2 Antibody Picoband

Gene Names

MT-CO2; COII; MTCO2; COX2

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U87 cells using anti-Nesprin 2/SYNE2 antibody (AAA19782).Overlay histogram showing U87 cells stained with AAA19782 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin 2/SYNE2 Antibody (AAA19782, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (AAA19782).Nesprin 2/SYNE2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin 2/SYNE2 Antibody (AAA19782) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin 2/SYNE2 Antibody (AAA19782) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin 2/SYNE2 Antibody (AAA19782) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human placenta tissue lysates,Lane 3: monkey COS-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin 2/SYNE2 antigen affinity purified polyclonal antibody (#AAA19782) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nesprin 2/SYNE2 at approximately 50-60 kDa. The expected band size for Nesprin 2/SYNE2 is at 796 kDa.)

Nesprin 2/SYNE2, Polyclonal Antibody (Cat# AAA19782)

Full Name

Anti-Nesprin 2/SYNE2 Antibody Picoband

Gene Names

SYNE2; NUA; EDMD5; Nesp2; TROPH; NUANCE; SYNE-2; Nesprin-2

Reactivity

Human, Monkey

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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TFEB, Polyclonal Antibody (Cat# AAA19403)

Full Name

Anti-TFEB Antibody Picoband

Gene Names

TFEB; TCFEB; BHLHE35; ALPHATFEB

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TOLLIP antibody. Blue: DAPI for nuclear staining.)

TOLLIP, Polyclonal Antibody (Cat# AAA10741)

Full Name

TOLLIP Polyclonal Antibody

Gene Names

TOLLIP; IL-1RAcPIP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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HNRNPA2B1, Polyclonal Antibody (Cat# AAA10693)

Full Name

HNRNPA2B1 Polyclonal Antibody

Gene Names

HNRNPA2B1; RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)

RPS7, Monoclonal Antibody (Cat# AAA24345)

Full Name

RPS7 (40S Ribosomal Protein S7) (AP)

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)

CSTF3, Monoclonal Antibody (Cat# AAA25068)

Full Name

CSTF3 (Cleavage Stimulation Factor Subunit 3, CF-1 77kD Subunit, Cleavage Stimulation Factor 77kD Subunit, CSTF 77kD Subunit, CstF-77) (FITC)

Gene Names

CSTF3; CSTF-77

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA25163)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (FITC)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of lysate from HepG2 cell line using PPT1 Antibody. AAA28640 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

PPT1, Monoclonal Antibody (Cat# AAA28640)

Full Name

PPT1 Antibody

Gene Names

PPT1; PPT; CLN1; INCL

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Mouse Monoclonal Antibody (Mab)

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Caspase-7/CASP7 antibody (AAA19232).Overlay histogram showing MCF-7 cells stained with AAA19232 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-7/CASP7 Antibody (AAA19232, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Caspase-7/CASP7, Polyclonal Antibody (Cat# AAA19232)

Full Name

Anti-Caspase-7/CASP7 Antibody

Gene Names

CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of SiHa cells using anti-Glutaminase/GLS antibody (AAA19239).Overlay histogram showing SiHa cells stained with AAA19239 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaminase/GLS Antibody (AAA19239,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Glutaminase/GLS, Polyclonal Antibody (Cat# AAA19239)

Full Name

Anti-Glutaminase/GLS Antibody

Gene Names

GLS; GAC; GAM; KGA; GLS1; AAD20

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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Cyclin A2, Monoclonal Antibody (Cat# AAA28470)

Full Name

Cyclin A2 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-IDUA antibody (AAA19772).Overlay histogram showing SH-SY5Y cells stained with AAA19772 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDUA Antibody (AAA19772, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of IDUA using anti-IDUA antibody (AAA19772).IDUA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human U87 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDUA antigen affinity purified polyclonal antibody (#AAA19772) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDUA at approximately 73 kDa. The expected band size for IDUA is at 73 kDa.)

IDUA, Polyclonal Antibody (Cat# AAA19772)

Full Name

Anti-IDUA Antibody Picoband

Gene Names

IDUA; IDA; MPS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U2OS cells using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Overlay histogram showing U2OS cells stained with AAA19716 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Sorbitol Dehydrogenase/SORD Antibody (AAA19716, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Sorbitol Dehydrogenase/SORD, Monoclonal Antibody (Cat# AAA19716)

Full Name

Anti-Sorbitol Dehydrogenase/SORD Antibody Picoband (monoclonal, 12B10G2)

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

EGF, ELISA Kit (Cat# AAA17891)

Full Name

Rat EGF ELISA Kit

Reactivity

Rat

Applications

Sandwich ELISA

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TXN2 antibody. Blue: DAPI for nuclear staining.)

TXN2, Polyclonal Antibody (Cat# AAA28253)

Full Name

TXN2 Polyclonal Antibody

Gene Names

TXN2; TXN; MTRX; TRX2; MT-TRX; COXPD29

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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XRCC6, Polyclonal Antibody (Cat# AAA10642)

Full Name

XRCC6 Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Affinity Purification

Pricing

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WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)

ACTB, Monoclonal Antibody (Cat# AAA26258)

Full Name

ACTB (Actin, beta, PS1TP5BP1) (Biotin)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)

SAV1, Monoclonal Antibody (Cat# AAA24044)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45 kDa WW Domain Protein, WW45, hWW45, WWP4)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HEL cells using anti-Annexin-4/ANXA4 antibody (AAA19529).Overlay histogram showing HEL cells stained with AAA19529 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Annexin-4/ANXA4, Polyclonal Antibody (Cat# AAA19529)

Full Name

Anti-Annexin-4/ANXA4 Antibody Picoband

Gene Names

ANXA4; ANX4; PIG28; ZAP36; HEL-S-274

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (RNPS1 monoclonal antibody (M05), clone 7G8. Western Blot analysis of RNPS1 expression in rat testis.)

RNPS1, Monoclonal Antibody (Cat# AAA25988)

Full Name

RNPS1 (RNA Binding Protein S1, Serine-Rich Domain, E5.1, MGC117332) (AP)

Gene Names

RNPS1; E5.1

Applications

Western Blot

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RELA antibody.)

p65, Polyclonal Antibody (Cat# AAA10711)

Full Name

p65 Polyclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROD1 on HeLa cell. [antibody concentration 20ug/ml])

ROD1, Monoclonal Antibody (Cat# AAA24932)

Full Name

ROD1 (PTBP3, Polypyrimidine Tract-binding Protein 3, Regulator of Differentiation 1) (Biotin)

Gene Names

PTBP3; ROD1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CD274 antibody. Blue: DAPI for nuclear staining.)

CD274, Polyclonal Antibody (Cat# AAA28238)

Full Name

CD274 Polyclonal Antibody

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

RBM9, Monoclonal Antibody (Cat# AAA24929)

Full Name

RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA) (Biotin)

Gene Names

RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

ERCC1/RAD10, Monoclonal Antibody (Cat# AAA23913)

Full Name

ERCC1/RAD10 (Tumor Progression Marker)

Gene Names

ERCC1; UV20; COFS4; RAD10

Reactivity

Human

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U2OS cells using anti-EIF4B antibody (AAA19408).Overlay histogram showing U2OS cells stained with AAA19408 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4B Antibody (AAA19408, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

EIF4B, Polyclonal Antibody (Cat# AAA19408)

Full Name

Anti-EIF4B Antibody Picoband

Gene Names

EIF4B; EIF-4B; PRO1843

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA25309)

Full Name

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (HRP)

Gene Names

AFP; AFPD; FETA; HPAFP

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Transketolase/TKT, Monoclonal Antibody (Cat# AAA19367)

Full Name

Anti-Transketolase/TKT Antibody (monoclonal, 3E5)

Gene Names

TKT; TK; TKT1; HEL107

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HL-60 cells using anti-REA/PHB2 antibody (AAA19273).Overlay histogram showing HL-60 cells stained with AAA19273 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-REA/PHB2 Antibody (AAA19273,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

REA/PHB2, Polyclonal Antibody (Cat# AAA19273)

Full Name

Anti-REA/PHB2 Antibody

Gene Names

PHB2; BAP; REA; p22; Bap37; BCAP37; PNAS-141

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-NOX2/gp91phox/CYBB antibody (AAA19218).Overlay histogram showing THP-1 cells stained with AAA19218 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX2/gp91phox/CYBB Antibody (AAA19218, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NOX2/gp91phox/CYBB, Polyclonal Antibody (Cat# AAA19218)

Full Name

Anti-NOX2/gp91phox/CYBB Antibody

Gene Names

CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-SNRNP70 antibody (AAA19791).Overlay histogram showing HepG2 cells stained with AAA19791 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP70 Antibody (AAA19791, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SNRNP70 using anti-SNRNP70 antibody (AAA19791) and anti-Beta Tubulin antibody (M01857-3).SNRNP70 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP70 antigen affinity purified polyclonal antibody (#AAA19791) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SNRNP70 at approximately 95 kDa. The expected band size for SNRNP70 is at 52 kDa.)

SNRNP70, Polyclonal Antibody (Cat# AAA19791)

Full Name

Anti-SNRNP70 Antibody Picoband

Gene Names

SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Histone H1.0/H1F0, Monoclonal Antibody (Cat# AAA19720)

Full Name

Anti-Histone H1.0/H1F0 Antibody Picoband (monoclonal, 5I3E6)

Gene Names

H1F0; H10; H1FV

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IFI16, Polyclonal Antibody (Cat# AAA28269)

Full Name

IFI16 Polyclonal Antibody

Gene Names

IFI16; PYHIN2; IFNGIP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA24571)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (APC)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 600ug extracts of 293T cells using 3ug NPM1 antibody . Western blot was performed from the immunoprecipitate using NPM1 antibody at a dilition of 1:1000.)

NPM1, Polyclonal Antibody (Cat# AAA28362)

Full Name

NPM1 Rabbit pAb

Gene Names

NPM1; B23; NPM

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-TARS2 antibody (AAA19627).Overlay histogram showing SiHa cells stained with AAA19627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TARS2 Antibody (AAA19627, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TARS2, Polyclonal Antibody (Cat# AAA19627)

Full Name

Anti-TARS2 Antibody Picoband

Gene Names

TARS2; thrRS; TARSL1; COXPD21

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of ASAH1 using anti-ASAH1 antibody.)

ASAH1, Polyclonal Antibody (Cat# AAA19197)

Full Name

Anti-ASAH1 Antibody

Gene Names

ASAH1; AC; PHP; ASAH; PHP32; ACDase; SMAPME

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M16), clone X1.Lane 1: FTL transfected lysate(20 KDa).Lane 2: Non-transfected lysate.)

FTL, Monoclonal Antibody (Cat# AAA26144)

Full Name

FTL (Ferritin, Light Polypeptide, MGC71996) (APC)

Gene Names

FTL; LFTD; NBIA3

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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IHC (Immunohistochemistry) (Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Lung Cancer Tissue)

Insulin receptor substrate 1, Polyclonal Antibody (Cat# AAA11632)

Full Name

Anti-IRS1 Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

eNOS, Monoclonal Antibody (Cat# AAA28545)

Full Name

eNOS Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U937 cells using anti-Syntaxin 18/STX18 antibody (AAA19967).Overlay histogram showing U937 cells stained with AAA19967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (AAA19967).Syntaxin 18/STX18 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 18/STX18 antigen affinity purified polyclonal antibody (#AAA19967) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Syntaxin 18/STX18 at approximately 39 kDa. The expected band size for Syntaxin 18/STX18 is at 39 kDa.)

Syntaxin 18/STX18, Polyclonal Antibody (Cat# AAA19967)

Full Name

Anti-Syntaxin 18/STX18 Antibody Picoband

Gene Names

STX18; Ufe1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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10000 results for "Recombinant Proteins" - showing 250-300

SAA1, ELISA Kit (Cat# AAA27871)

SMAD3, Monoclonal Antibody (Cat# AAA26429)

SCG10/STMN2, Polyclonal Antibody (Cat# AAA19299)

APLP2, Polyclonal Antibody (Cat# AAA19746)

Filamin B/FLNB, Polyclonal Antibody (Cat# AAA19463)

ACTR2, Polyclonal Antibody (Cat# AAA28152)

Cytokeratin 14 (KRT14), Monoclonal Antibody (Cat# AAA28471)

ASC/TMS1/PYCARD, Polyclonal Antibody (Cat# AAA19733)

MT-CO2, Polyclonal Antibody (Cat# AAA19493)

Nesprin 2/SYNE2, Polyclonal Antibody (Cat# AAA19782)

TFEB, Polyclonal Antibody (Cat# AAA19403)

TOLLIP, Polyclonal Antibody (Cat# AAA10741)

HNRNPA2B1, Polyclonal Antibody (Cat# AAA10693)

RPS7, Monoclonal Antibody (Cat# AAA24345)

CSTF3, Monoclonal Antibody (Cat# AAA25068)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA25163)

PPT1, Monoclonal Antibody (Cat# AAA28640)

Caspase-7/CASP7, Polyclonal Antibody (Cat# AAA19232)

Glutaminase/GLS, Polyclonal Antibody (Cat# AAA19239)

Cyclin A2, Monoclonal Antibody (Cat# AAA28470)

IDUA, Polyclonal Antibody (Cat# AAA19772)

Sorbitol Dehydrogenase/SORD, Monoclonal Antibody (Cat# AAA19716)

EGF, ELISA Kit (Cat# AAA17891)

TXN2, Polyclonal Antibody (Cat# AAA28253)

XRCC6, Polyclonal Antibody (Cat# AAA10642)

ACTB, Monoclonal Antibody (Cat# AAA26258)

SAV1, Monoclonal Antibody (Cat# AAA24044)

Annexin-4/ANXA4, Polyclonal Antibody (Cat# AAA19529)

RNPS1, Monoclonal Antibody (Cat# AAA25988)

p65, Polyclonal Antibody (Cat# AAA10711)

ROD1, Monoclonal Antibody (Cat# AAA24932)

CD274, Polyclonal Antibody (Cat# AAA28238)

RBM9, Monoclonal Antibody (Cat# AAA24929)

ERCC1/RAD10, Monoclonal Antibody (Cat# AAA23913)

EIF4B, Polyclonal Antibody (Cat# AAA19408)

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA25309)

Transketolase/TKT, Monoclonal Antibody (Cat# AAA19367)

REA/PHB2, Polyclonal Antibody (Cat# AAA19273)

NOX2/gp91phox/CYBB, Polyclonal Antibody (Cat# AAA19218)

SNRNP70, Polyclonal Antibody (Cat# AAA19791)

Histone H1.0/H1F0, Monoclonal Antibody (Cat# AAA19720)

IFI16, Polyclonal Antibody (Cat# AAA28269)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA24571)

NPM1, Polyclonal Antibody (Cat# AAA28362)

TARS2, Polyclonal Antibody (Cat# AAA19627)

ASAH1, Polyclonal Antibody (Cat# AAA19197)

FTL, Monoclonal Antibody (Cat# AAA26144)

Insulin receptor substrate 1, Polyclonal Antibody (Cat# AAA11632)

eNOS, Monoclonal Antibody (Cat# AAA28545)

Syntaxin 18/STX18, Polyclonal Antibody (Cat# AAA19967)

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