Search results for "Recombinant Proteins"

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WB (Western Blot)

Ezrin/p81, Monoclonal Antibody (Cat# AAA214832)

Full Name

Ezrin/p81

Gene Names

EZR; CVL; CVIL; VIL2; HEL-S-105

Reactivity

Human. Others not tested.

Applications

Immunohistochemistry, Western Blot

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IHC (Immunohistochemistry) (Figure 4. IHC analysis of LY6C using anti-LY6C antibody (AAA125830).LY6C was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LY6C Antibody (AAA125830) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

LY6C, Polyclonal Antibody (Cat# AAA125830)

Full Name

Anti-LY6C Antibody

Gene Names

Ly6c1; Ly6c; Ly-6C; Ly-6C1; AA682074; AA959465

Reactivity

Mouse, Rat

Applications

Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U20S cells using anti-P Glycoprotein/ABCB1 antibody (AAA125513).Overlay histogram showing U20S cells stained with AAA125513 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein/ABCB1 Antibody (AAA125513, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

P Glycoprotein/ABCB1, Polyclonal Antibody (Cat# AAA125513)

Full Name

Anti-P Glycoprotein/ABCB1 Antibody

Gene Names

ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170

Reactivity

Human, Mouse, Rat

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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pyruvate dehydrogenase (lipoamide) alpha 1, Monoclonal Antibody (Cat# AAA320308)

Full Name

pyruvate dehydrogenase (lipoamide) alpha 1 Mouse mAb

Gene Names

PDHA1; PDHA; PHE1A; PDHCE1A

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Pricing

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Laminin Alpha 3 (LAMa3), Monoclonal Antibody (Cat# AAA151788)

Full Name

Monoclonal Antibody to Laminin Alpha 3 (LAMa3)

Gene Names

LAMA3; E170; LOCS; BM600; LAMNA; lama3a

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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Bioactivity (Interleukin 1 alpha (IL-1alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL-1alphais produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. To test the effect of IL-1alpha on cell apoptosis, MCF7 cells were seeded into 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant goat IL-1alpha. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37 degree C. Proliferation of MCF7 cells after incubation with IL-1alpha for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant goat IL-1alpha for 72h. The result was shown in Figure 2. It was obvious that IL-1alpha significantly inhibit cell viability of MCF7cells. The ED50 is 2.13 ug/ml.)

Interleukin 1 Alpha (IL1a), Active Protein (Cat# AAA161676)

Full Name

Active Interleukin 1 Alpha (IL1a)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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IP (Immunoprecipitation) (YWHAE was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-YWHAE mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAE mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa)

14-3-3 epsilon, Monoclonal Antibody (Cat# AAA258853)

Full Name

Anti-14-3-3 epsilon Antibody, Mouse Monoclonal

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A

Pricing

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IP (Immunoprecipitation) (ALPL was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti- ALPL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- ALPL rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57kDa Observed band size :80 kDa)

Alkaline Phosphatase/ALPL, Polyclonal Antibody (Cat# AAA258515)

Full Name

Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Polyclonal

Gene Names

TGFBR1; AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4

Reactivity

Human

Applications

ELISA

Purity

Protein A & Antigen Affinity

Pricing

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ZAP-70, Monoclonal Antibody (Cat# AAA320376)

Full Name

ZAP-70 Mouse mAb

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Pricing

KIM1/TIM1, ELISA Kit (Cat# AAA125881)

Full Name

Mouse KIM1/TIM1 Quick ELISA Kit

Gene Names

Havcr1; Tim1; KIM-1; TIM-1; Timd1; AI503787

Reactivity

Mouse

Pricing

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IF (Immunofluorescence) (Figure 2. IF analysis of Calpain 6/CAPN6 using anti-Calpain 6/CAPN6 antibody (AAA128231).Calpain 6/CAPN6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4ug/mL rabbit anti-Calpain 6/CAPN6 Antibody (AAA128231) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Calpain 6/CAPN6, Polyclonal Antibody (Cat# AAA128231)

Full Name

Anti-Calpain 6/CAPN6 Antibody Picoband

Gene Names

CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Lung cancer Tissue; Primary Ab: 20ug/ml Mouse AntiHuman CASP9 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Caspase 9 (CASP9), Monoclonal Antibody (Cat# AAA151588)

Full Name

Monoclonal Antibody to Caspase 9 (CASP9)

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of PC-3 cells using anti-CEA/CEACAM5 antibody (AAA126981).Overlay histogram showing PC-3 cells stained with AAA126981 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CEA/CEACAM5 Antibody (AAA126981, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CEA/CEACAM5, Polyclonal Antibody (Cat# AAA126981)

Full Name

Anti-CEA/CEACAM5 Antibody Picoband

Gene Names

CEACAM5; CEA; CD66e

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Bioactivity (Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)

Bioactivity (G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. The activity of G-CSF is usually measured by a cell proliferation assay using M-NFS60 mouse myelogenous leukemia lymphoblast cells. M-NFS60 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant human G-CSF. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 degree C. Proliferation of M-NFS-60 cells after incubation with G-CSF for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant human G-CSF for 3 days. The result was shown in Figure 2. It was obvious that G-CSF significantly increased cell viability of M-NFS-60 cells. The EC50 is 0.039 ug/ml.)

Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161662)

Full Name

Active Colony Stimulating Factor 3, Granulocyte (GCSF)

Gene Names

CSF3; GCSF; CSF3OS; C17orf33

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of HEL cells using anti-CXCR5 antibody (AAA126054).Overlay histogram showing HEL cells stained with AAA126054 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR5 Antibody (AAA126054, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CXCR5, Polyclonal Antibody (Cat# AAA126054)

Full Name

Anti-CXCR5 Antibody Picoband

Gene Names

CXCR5; BLR1; CD185; MDR15

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of JK cells using anti-H-ERG/KCNH2 antibody (AAA126075).Overlay histogram showing JK cells stained with AAA126075 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-H-ERG/KCNH2 Antibody (AAA126075, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

H-ERG/KCNH2, Polyclonal Antibody (Cat# AAA126075)

Full Name

Anti-H-ERG/KCNH2 Antibody Picoband

Gene Names

KCNH2; ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 4. IHC analysis of BUB1 using anti-BUB1 antibody (AAA126073).BUB1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-BUB1 Antibody (AAA126073) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

BUB1, Polyclonal Antibody (Cat# AAA126073)

Full Name

Anti-BUB1 Antibody Picoband

Gene Names

BUB1; BUB1A; BUB1L; hBUB1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA126911).CD13/ANPEP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD13/ANPEP Antibody (AAA126911) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

CD13/ANPEP, Monoclonal Antibody (Cat# AAA126911)

Full Name

Anti-CD13/ANPEP Antibody Picoband (monoclonal, 7F2)

Gene Names

ANPEP; APN; CD13; LAP1; P150; PEPN; GP150

Reactivity

Human, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 5. IHC analysis of FHL2 using anti-FHL2 antibody (AAA126201).FHL2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FHL2 Antibody (AAA126201) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

FHL2, Polyclonal Antibody (Cat# AAA126201)

Full Name

Anti-FHL2 Antibody Picoband

Gene Names

FHL2; DRAL; AAG11; FHL-2; SLIM3; SLIM-3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Bioactivity (Small calcium binding protein that S100 Calcium Binding Protein (S100), also known as S100 Alpha (S100A1), is a member of the S100 family of calcium-binding proteins. As with most S100 proteins, S100A1 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and it possess a variety of intracellular and extracellular functions. They interact with multiple receptors and signal transducers to regulate pathways that govern inflammation, cell differentiation, proliferation, energy metabolism, apoptosis, calcium homeostasis, cell cytoskeleton and microbial resistance. S100 Calcium Binding Protein A4 (S100A4) is one of targets of S100A1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human S100A1 and recombinant mouse S100A4. Briefly, S100A1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100A4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human S100A1 and recombinant mouse S100A4 was shown in Figure 1, the EC50 for this effect is 0.11 ug/mL.)

S100 Calcium Binding Protein (S100), Active Protein (Cat# AAA161650)

Full Name

Active S100 Calcium Binding Protein (S100)

Gene Names

S100A1; S100; S100A; S100-alpha

Applications

Activity Assay, Cell Culture

Purity

Greater than 97% by SDS-PAGE

Pricing

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Wolfram Syndrome Protein 1 (WFS1), Recombinant Protein (Cat# AAA150764)

Full Name

Recombinant Wolfram Syndrome Protein 1 (WFS1)

Gene Names

WFS1; WFS; WFRS; WFSL; CTRCT41

Applications

Western Blot

Purity

>95%

Pricing

BMP-5, ELISA Kit (Cat# AAA124760)

Full Name

Horse equine BMP-5 PicoKine ELISA Kit

Reactivity

Equine
There is no detectable cross-reactivity with other relevant proteins.

Applications

ELISA

Pricing

IL-17F, ELISA Kit (Cat# AAA124973)

Full Name

Human IL-17F PicoKine ELISA Kit

Gene Names

IL17F; ML1; ML-1; CANDF6; IL-17F

Reactivity

Human
There is no detectable cross-reactivity with other relevant proteins.

Pricing

CD61/ITGB3, Recombinant Protein (Cat# AAA120434)

Full Name

Recombinant Human CD61/ITGB3, C-Strep

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

Full Name

IP-10 (Human) LumiAb ELISA Kit

Gene Names

CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10

Reactivity

Human

Pricing

Survivin, ELISA Kit (Cat# AAA124801)

Full Name

Monkey primate Survivin PicoKine ELISA Kit

Gene Names

BIRC5; API4; EPR-1

Reactivity

Monkey
There is no detectable cross-reactivity with other relevant proteins.

Applications

ELISA

Pricing

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Full Name

LIN28A Monoclonal Antibody

Gene Names

LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity purified

Pricing

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SDS-PAGE (Figure. SDS-PAGE)

Protein Kinase, AMP Activated Beta 1 (PRKAb1), Recombinant Protein (Cat# AAA150690)

Full Name

Recombinant Protein Kinase, AMP Activated Beta 1 (PRKAb1)

Applications

Western Blot

Purity

>90%

Pricing

Thrombomodulin, ELISA Kit (Cat# AAA315834)

Full Name

Thrombomodulin (Human) OmniKine ELISA Kit

Gene Names

THBD; TM; THRM; AHUS6; BDCA3; CD141; THPH12

Reactivity

Human

Pricing

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Catechol-O-Methyltransferase (COMT), Recombinant Protein (Cat# AAA150758)

Full Name

Recombinant Catechol-O-Methyltransferase (COMT)

Gene Names

Comt; Comt1; D16Wsu103e; D330014B15Rik

Applications

Western Blot

Purity

>95%

Pricing

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Bioactivity (HTRF assay for WNK2 (1-489); activity 1 uM STK S3 substrate was incubated with different concentrations of WNK2 (1-489); protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.)