Standard Curve (Sample)
General PAI-1 functional assay ELISA Kit | SERPINE1 elisa kit
Active mouse PAI-1 functional assay ELISA Kit
Gene Names
Serpine1; PAI1; PAI-1; Planh1
Reactivity
General
Synonyms
PAI-1 functional assay; N/A; Active mouse PAI-1 functional assay ELISA Kit; SERPINE1 elisa kit
Reactivity
General
Specificity
This assay recognizes natural and recombina nt active mouse PAI-l. Pooled normal plasma from human, rat, pig, sheep, canine, and guinea pig were assayed and no significant cross-reactivity was observed. Pooled normal plasma from rabbit resu lted in significant color development. The following factors were prepared in blocking buffer and assayed for cross-reactivity.
Form/Format
Complete Kit
Sequence Length
402
Samples
Mouse plasma
Sensitivity
0.05 ng/ml
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
These studies are currently in progress. Please cont act us for more information.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SERPINE1 elisa kit
Background: Plasminogen activator inhibitor-1 (PAI-l) is a central regu lator of the blood fibrinolytic system [2). Clinical studies have indicated that increased PAI-l levels increase the risk for thrombosis, whereas decreased levels may cause recu rrent bleeding [3).
Principle of the Assay: Functionally active PAI-l present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, polyclonal anti-mouse PAI-l primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active PAI-l in the samples. A standard ca libration curve is prepared along with the samples to be measured using dilutions of mouse PAI-1.
Intended Uses: Mouse PAI-l activity assay is intended for the quantitative determination of active plasminogen activat or inhibitor type 1 in mouse plasma. For research use only.
Principle of the Assay: Functionally active PAI-l present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, polyclonal anti-mouse PAI-l primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active PAI-l in the samples. A standard ca libration curve is prepared along with the samples to be measured using dilutions of mouse PAI-1.
Intended Uses: Mouse PAI-l activity assay is intended for the quantitative determination of active plasminogen activat or inhibitor type 1 in mouse plasma. For research use only.
Product Categories/Family for SERPINE1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,170 Da
NCBI Official Full Name
Plasminogen activator inhibitor 1
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade E, member 1
NCBI Official Symbol
Serpine1
NCBI Official Synonym Symbols
PAI1; PAI-1; Planh1
NCBI Protein Information
plasminogen activator inhibitor 1; PAI; serpin E1; plasminogen activator inhibitor, type I; endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI)
UniProt Protein Name
Plasminogen activator inhibitor 1
UniProt Gene Name
Serpine1
UniProt Synonym Gene Names
Mr1; Pai1; Planh1; PAI; PAI-1
UniProt Entry Name
PAI1_MOUSE
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Product Notes
The General SERPINE1 serpine1 (Catalog #AAA37759) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37759 ELISA Kit recognizes General SERPINE1. It is sometimes possible for the material contained within the vial of "PAI-1 functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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