Bioactivity
(Human SIRP alpha/CD172a is coated at 2 ug/ml (100 ul/well) can bind Human CD47 in a Functional ELISA assay.)
SIRP alpha/CD172a Recombinant Protein | SIRPalpha/CD172a recombinant protein
Recombinant human SIRP alpha/CD172a protein
Gene Names
SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SIRP alpha/CD172a; N/A; Recombinant human SIRP alpha/CD172a protein; SIRPA; Tyrosine-protein phosphatase non-receptor type substrate 1 isoform 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD172a; BIT; MFR; MYD1; PTPNS1; SHPS1;SIRP; SIRPalpha/CD172a recombinant protein
Host
HEK293
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
27-373aa
Sequence
Applicable Applications for SIRPalpha/CD172a recombinant protein
Bioactivity, SDS-PAGE
Species
Human
Tag
His-Tag
Predicted Nterm
Asp
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD47
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Human SIRP alpha/CD172a is coated at 2 ug/ml (100 ul/well) can bind Human CD47 in a Functional ELISA assay.)
Bioactivity
(Human SIRP alpha/CD172a is coated at 2 ug/ml (100 ul/well) can bind Human CD47 in a Functional ELISA assay.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for SIRPalpha/CD172a recombinant protein
SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha interacts with a broadly expressed transmembrane protein CD47. This interaction negatively controls effector function of innate immune cells such as host cell phagocytosis. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. It was found to participate in signal transduction mediated by various growth factor receptors. Recombinant human SIRP alpha, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Categories/Family for SIRPalpha/CD172a recombinant protein
References
Timms J.F., Swanson K.D., et al. (1999) Curr. Biol. 9:927-930.;; Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,967 Da
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type substrate 1
NCBI Official Synonym Full Names
signal-regulatory protein alpha
NCBI Official Symbol
SIRPA
NCBI Official Synonym Symbols
BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; OTTHUMP00000030001; OTTHUMP00000159968; OTTHUMP00000159969; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP sub
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type substrate 1
UniProt Gene Name
SIRPA
UniProt Synonym Gene Names
BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2
UniProt Entry Name
SHPS1_HUMAN
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Product Notes
The SIRPalpha/CD172a sirpa (Catalog #AAA48418) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 27-373aa. AAA Biotech's SIRP alpha/CD172a can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the SIRPalpha/CD172a sirpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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