SDS-PAGE
(The purity of SMAD9 was determined to be >70% by densitometryApprox. MW86kDa)
SMAD9 recombinant protein
SMAD9 recombinant protein
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
Applications
Western Blot
Purity
>70%
Synonyms
SMAD9; N/A; SMAD9 recombinant protein; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
Host
E Coli
Purity/Purification
>70%
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.2ug/ul (varies by lot)
Sequence Length
5592
Applicable Applications for SMAD9 recombinant protein
WB (Western Blot)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant full-length human SMAD9 was expressed by baculovirus in E.coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with Dry Ice.
Shipping: Ships with Dry Ice.
SDS-PAGE
(The purity of SMAD9 was determined to be >70% by densitometryApprox. MW86kDa)
SDS-PAGE
(The purity of SMAD9 was determined to be >70% by densitometryApprox. MW86kDa)
Related Product Information for SMAD9 recombinant protein
Recombinant full-length human SMAD9 was expressed by baculovirus in E.coli cells using an N-terminal GST tag.
Scientific Background: SMAD9 or SMAD family member 9 is a member of the SMAD family. SMAD9 transduces signals from TGF-beta family members which regulate growth, differentiation, apoptosis, and development. SMAD9 is activated by bone morphogenetic proteins and it interacts with SMAD4 (1). SMAD9 positively and negatively regulate BMP signaling, respectively. SMAD9, also known as SMAD8 may play a role in both pulmonary hypertension and lung tumorigenesis (2). Mutations in SMAD9 in patient with pulmonary arterial hypertension (PAH) have been identified and this may be involved in the pathogenesis of PAH.
Scientific Background: SMAD9 or SMAD family member 9 is a member of the SMAD family. SMAD9 transduces signals from TGF-beta family members which regulate growth, differentiation, apoptosis, and development. SMAD9 is activated by bone morphogenetic proteins and it interacts with SMAD4 (1). SMAD9 positively and negatively regulate BMP signaling, respectively. SMAD9, also known as SMAD8 may play a role in both pulmonary hypertension and lung tumorigenesis (2). Mutations in SMAD9 in patient with pulmonary arterial hypertension (PAH) have been identified and this may be involved in the pathogenesis of PAH.
Product Categories/Family for SMAD9 recombinant protein
References
1. Nishita, M. et.al: Smad8B, a Smad8 splice variant lacking the SSXS site that inhibits Smad8-mediated signalling. Genes Cells 4: 583-591, 1999.
2. Huang, Z. et.al: Defective pulmonary vascular remodeling in Smad8 mutant mice. Hum. Molec. Genet. 18: 2791-2801, 2009.
2. Huang, Z. et.al: Defective pulmonary vascular remodeling in Smad8 mutant mice. Hum. Molec. Genet. 18: 2791-2801, 2009.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~86 kDa
NCBI Official Full Name
Homo sapiens SMAD family member 9 (SMAD9), transcript variant a, mRNA
NCBI Official Synonym Full Names
SMAD family member 9
NCBI Official Symbol
SMAD9
NCBI Official Synonym Symbols
PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
NCBI Protein Information
mothers against decapentaplegic homolog 9; MAD homolog 9; SMAD, mothers against DPP homolog 9; Mothers against decapentaplegic, drosophila, homolog of, 9
UniProt Protein Name
Mothers against decapentaplegic homolog 9
UniProt Gene Name
SMAD9
UniProt Synonym Gene Names
MADH6; MADH9; MAD homolog 9; Mothers against DPP homolog 9; SMAD 9; Smad9
UniProt Entry Name
SMAD9_HUMAN
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Product Notes
The SMAD9 smad9 (Catalog #AAA72914) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||E.coli!!Source Note||Recombinant full-length human SMAD9 was expressed by baculovirus in E.coli cells. AAA Biotech's SMAD9 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SMAD9 smad9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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