Rapid Dewaxing Solution
One-step Rapid Dewaxing Solution
Synonyms
Rapid Dewaxing; N/A; One-step Rapid Dewaxing Solution; Rapid Dewaxing solution
Instruction
Dewaxing solution (10×) is a 10× concentrated solution. Dilute with ddH20 to 1 ×Dewaxing working solution before use.
Experimental Procedure
1. Dilute the dewaxing solution (10×) with ddH2O at the ratio of 1:9.
Note: Flocculent sediment will be generated during dilution. Do not blow it with any pipette. Please bake the diluted solution at 60 degree C in an oven for 30 min, then stirred or blown to mix evenly, and finally the solution present as milky white suspension.
2. Dewax the slides with 1× Dewaxing solution. Rewarm the dewaxing solution at 60 degree C for 30 min, immerse the slides into the Dewaxing solution and incubate at 60 degree C for 30 min.
Note: Low temperature may affect the effect of dewaxing. Please rewarm the dewaxing solution at 60 degree C for 30 min before the dewaxing. If the paraffin slides are thick, you can dewax the slides for 50 min.
3. Wash the slides with tap water for 5 min until froth free.
Note: Don't wash the tissue directly, avoid tissue damage.
4. Absorbs the moisture around the tissue with filter paper for the next step. Or immerse the slides in PBS for use.
5. For IHC experiment, follow the antigen repair step. For TUNEL or HE staining experiment, please follow the staining kit steps.
Note: Flocculent sediment will be generated during dilution. Do not blow it with any pipette. Please bake the diluted solution at 60 degree C in an oven for 30 min, then stirred or blown to mix evenly, and finally the solution present as milky white suspension.
2. Dewax the slides with 1× Dewaxing solution. Rewarm the dewaxing solution at 60 degree C for 30 min, immerse the slides into the Dewaxing solution and incubate at 60 degree C for 30 min.
Note: Low temperature may affect the effect of dewaxing. Please rewarm the dewaxing solution at 60 degree C for 30 min before the dewaxing. If the paraffin slides are thick, you can dewax the slides for 50 min.
3. Wash the slides with tap water for 5 min until froth free.
Note: Don't wash the tissue directly, avoid tissue damage.
4. Absorbs the moisture around the tissue with filter paper for the next step. Or immerse the slides in PBS for use.
5. For IHC experiment, follow the antigen repair step. For TUNEL or HE staining experiment, please follow the staining kit steps.
Preparation and Storage
Store at room temperature for 12 months.
Estimated Expiration: 12 months
Estimated Expiration: 12 months
Related Product Information for Rapid Dewaxing solution
Lab one step rapid dewaxing solution is a rapid reagent for paraffin slides pretreatment. There is no need to do the traditional three times of xylene dewaxing, three to five times of gradient ethanol hydration, but only one Dewaxing solution. The operation time is effectively shortened, the cumbersome operation steps are simplified so the variables in operation are reduced, and the stability is improved. The Dewaxing solution applies new environmental protection technology is not only reduces the harm to the body, but also cause no pollution to the environment.
Product Categories/Family for Rapid Dewaxing solution
Similar Products
Product Notes
The Rapid Dewaxing (Catalog #AAA178128) is a Solution and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Rapid Dewaxing, Solution" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.