IHC (Immunohistochemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IDO1 recombinant antibody
IDO1 Antibody
Gene Names
IDO1; IDO; INDO; IDO-1
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Affinity-chromatography
Synonyms
IDO1, Recombinant Antibody; IDO1 Antibody; Indoleamine 2;3-dioxygenase 1 (IDO-1) (EC 1.13.11.52) (Indoleamine-pyrrole 2;3-dioxygenase); IDO1; IDO INDO; IDO1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
9E5
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for IDO1 recombinant antibody
IHC (Immunohistochemistry), ELISA
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human INDO
Immunogen Species
Homo sapiens (Human)
Research Area
Cancer; Cardiovascular; Metabolism; Signal transduction
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IHC (Immunohistochemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Related Product Information for IDO1 recombinant antibody
Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).
Description: IDO1 is a heme enzyme that catalyzes the oxidation of L-tryptophan (L-Trp) into kynurenine metabolites, the buildup of which causes T-cell suppression and allows tumor cells to evade immune system monitoring and clearance. In vitro, IDO1 has been demonstrated to block T cell activation and promote the formation of T regulatory cells. In vivo, IDO1 is well known for facilitating tumor immune evasion through its immunoregulatory role. IDO1 is significantly expressed in a number of malignancies, including colorectal cancer, breast cancer, and esophageal carcinoma, and its overexpression has been linked to a bad prognosis in various cancers.
Genes for IDO1 antibody's heavy and light chains were cloned into plasma vectors, which were subsequently transfected into mammalian cells for expression. The resulting product is the recombinant IDO1 antibody. This recombinant IDO1 antibody was subsequently purified from the culture medium of transfected host cell lines through A synthesized peptide derived from human INDO. It has verified to detect IDO1 protein Human in the ELISA, IHC.
Description: IDO1 is a heme enzyme that catalyzes the oxidation of L-tryptophan (L-Trp) into kynurenine metabolites, the buildup of which causes T-cell suppression and allows tumor cells to evade immune system monitoring and clearance. In vitro, IDO1 has been demonstrated to block T cell activation and promote the formation of T regulatory cells. In vivo, IDO1 is well known for facilitating tumor immune evasion through its immunoregulatory role. IDO1 is significantly expressed in a number of malignancies, including colorectal cancer, breast cancer, and esophageal carcinoma, and its overexpression has been linked to a bad prognosis in various cancers.
Genes for IDO1 antibody's heavy and light chains were cloned into plasma vectors, which were subsequently transfected into mammalian cells for expression. The resulting product is the recombinant IDO1 antibody. This recombinant IDO1 antibody was subsequently purified from the culture medium of transfected host cell lines through A synthesized peptide derived from human INDO. It has verified to detect IDO1 protein Human in the ELISA, IHC.
Product Categories/Family for IDO1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
403
NCBI Official Full Name
Indoleamine 2,3-dioxygenase 1
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
IDO1
NCBI Official Synonym Symbols
IDO; INDO; IDO-1
NCBI Protein Information
indoleamine 2,3-dioxygenase 1; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Gene Name
IDO1
UniProt Synonym Gene Names
IDO; INDO; IDO-1
UniProt Entry Name
I23O1_HUMAN
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Product Notes
The IDO1 ido1 (Catalog #AAA243839) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IDO1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IDO1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the IDO1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDO1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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