Standard Curve (Sample)
Rat TIMP-1 ELISA Kit | TIMP1 elisa kit
Rat TIMP-1 PicoKine ELISA Kit
Gene Names
Timp1; Timp; TIMP-1
Reactivity
Rat
Synonyms
TIMP-1; N/A; Rat TIMP-1 PicoKine ELISA Kit; Metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CCR5_HUMAN; CD195; CHEMR13; CLGI; Collagenase inhibitor; EPA; EPO; Erythroid potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; HIV-1 fusion coreceptor; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP; TIMP 1; TIMP1 protein; Tissue Inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinases; Tissue inhibitor of metalloproteinases 1; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor; TIMP1 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Timp1
Sequence Length
217
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<3 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TIMP1 elisa kit
Background: The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32-34KDa.
Principle of the Assay: The Picokine™ Rat Timp1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Timp1 with a 96-well strip plate that is pre-coated with antibody specific for Timp1. The detection antibody is a biotinylated antibody specific for Timp1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Timp1 with immunogen: Expression system for standard: NS0; Immunogen sequence: C24-A217. The kit is analytically validated with ready-to-use reagents. To measure Rat Timp1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Timp1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Timp1 in the sample. Fo
Principle of the Assay: The Picokine™ Rat Timp1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Timp1 with a 96-well strip plate that is pre-coated with antibody specific for Timp1. The detection antibody is a biotinylated antibody specific for Timp1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Timp1 with immunogen: Expression system for standard: NS0; Immunogen sequence: C24-A217. The kit is analytically validated with ready-to-use reagents. To measure Rat Timp1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Timp1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Timp1 in the sample. Fo
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,794 Da
NCBI Official Full Name
Metalloproteinase inhibitor 1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
Timp1
NCBI Official Synonym Symbols
Timp; TIMP-1
NCBI Protein Information
metalloproteinase inhibitor 1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinases 1
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Gene Name
Timp1
UniProt Synonym Gene Names
Timp-1; TIMP-1
UniProt Entry Name
TIMP1_RAT
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Product Notes
The Rat TIMP1 timp1 (Catalog #AAA11569) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11569 ELISA Kit recognizes Rat TIMP1. It is sometimes possible for the material contained within the vial of "TIMP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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