Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC)
Transmembrane 4 L6 family member 1 (TM4SF1) Recombinant Protein | TM4SF1 recombinant protein
Recombinant Macaca fascicularis Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active)
Purity
The purity information is not available for VLPs proteins.
Synonyms
Transmembrane 4 L6 family member 1 (TM4SF1); N/A; Recombinant Macaca fascicularis Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active); TM4SF1 recombinant protein
Host
Mammalian cell
Purity/Purification
The purity information is not available for VLPs proteins.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Sequence Positions
1-202aa; Full Length
Sequence
MCYGKCARCIGHSLVGLALLCIIANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLLPAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDSFGQWNYTFASTEGQYLLDTSTWSQCTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGGICGFCCSRQQQYDC
Species
Macaca fascicularis (Crab-eating macaque)(Cynomolgus monkey)
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Research Area
CancerLess than 1.0 EU/ug as determined by LAL method.
Transmembrane Domain
4TM
Biological_Activity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus TM4SF1 at 5 ug/mL can bind Anti-TM4SF1 recombinant antibody.The EC50 is 4.480-4.930 ng/mL.The VLPs is negative control.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC)
Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Cynomolgus TM4SF1 at 5ug/mL can bind Anti-TM4SF1 recombinant antibody , the EC50 is 4.480-4.930 ng/mL.VLPs is negative control.)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Cynomolgus TM4SF1 at 5ug/mL can bind Anti-TM4SF1 recombinant antibody , the EC50 is 4.480-4.930 ng/mL.VLPs is negative control.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody.The two bands respectively correspond to monomer, Homodimer.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody.The two bands respectively correspond to monomer, Homodimer.)
Product Categories/Family for TM4SF1 recombinant protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
23.1kDa
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Product Notes
The TM4SF1 (Catalog #AAA279277) is a Recombinant Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-202aa; Full Length. The amino acid sequence is listed below: MCYGKCARCI GHSLVGLALL CIIANILLYF PNGETKYASE NHLSRFVWFF SGIVGGGLLM LLPAFVFIGL EQDDCCGCCG HENCGKRCAM LSSVLAALIG IAGSGYCVIV AALGLAEGPL CLDSFGQWNY TFASTEGQYL LDTSTWSQCT EPKHIVEWNV SLFSILLALG GIEFILCLIQ VINGVLGGIC GFCCSRQQQY DC. It is sometimes possible for the material contained within the vial of "Transmembrane 4 L6 family member 1 (TM4SF1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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