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product-image-AAA79703_SC15.png Standard Curve (Sample)

Human ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA Kit | UCHL1 elisa kit

Human Protein Gene Product 9.5, PGP 9.5 ELISA Kit

Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human
Synonyms
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); N/A; Human Protein Gene Product 9.5, PGP 9.5 ELISA Kit; Human Protein Gene Product 9.5 (PGP 9.5) ELISA Kit; PARK5; PGP9.5; PGP95; Uch-L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin carboxyl-terminal esterase L1; UCHL1 elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human PGP 9.5. No significant cross-reactivity or interference between human PGP 9.5 and analogues was observed.
Sequence Length
223
Samples
Serum, plasma, tissue homogenates, cerebrospinal fluid (CSF).
Assay Type
Sandwich (Quantitative)
Detection Range
6.25 ng/ml-400 ng/ml.
Sensitivity
1.56 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA79703_SC15.png Standard Curve (Sample)
Related Product Information for UCHL1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PGP 9.5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGP 9.5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGP 9.5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGP 9.5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,824 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN

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Product Notes

The Human UCHL1 uchl1 (Catalog #AAA79703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79703 ELISA Kit recognizes Human UCHL1. It is sometimes possible for the material contained within the vial of "ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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