SDS-PAGE
VEGF165/VEGFA recombinant protein
Recombinant Human VEGF165/VEGFA Protein (Active)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Purity
> 95% as determined by reducing SDS-PAGE.
Synonyms
VEGF165/VEGFA; N/A; Recombinant Human VEGF165/VEGFA Protein (Active); MVCD1; VEGF; VEGF165; VPF; Vascular Endothelial Growth Factor Isoform 165; VEGF165/VEGFA recombinant protein
Host
Human Cells
Purity/Purification
> 95% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Sequence
Ala27-Arg191
Sequence Length
171
Species
Human
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Bio_activity
Immobilized Human VEGF165 at 10ug/ml (100 ul/well) can bind Human VEGFR2, the ED50 of Human VEGF165 is 433.6 ng/ml.
Reconstitution
We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.
It is recommended that 50mM Acetic acid (250ul) be added to the vial to prepare a stock solution of 0.2 mg/mL. Concentration is measured by UV-Vis.
It is recommended that 50mM Acetic acid (250ul) be added to the vial to prepare a stock solution of 0.2 mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
SDS-PAGE
SDS-PAGE
Related Product Information for VEGF165/VEGFA recombinant protein
Protein Construction:
Recombinant Human VEGF165 is produced by our mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.
Background:
Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
Recombinant Human VEGF165 is produced by our mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.
Background:
Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Mass: 19.1kDa
Actual Protein Molecular Mass: 18-22kDa
Actual Protein Molecular Mass: 18-22kDa
NCBI Official Full Name
vascular endothelial growth factor A isoform q
NCBI Official Synonym Full Names
vascular endothelial growth factor A
NCBI Official Symbol
VEGFA
NCBI Official Synonym Symbols
VPF; VEGF; MVCD1
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
UniProt Gene Name
VEGFA
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF
UniProt Entry Name
VEGFA_HUMAN
Similar Products
Product Notes
The VEGF165/VEGFA vegfa (Catalog #AAA174692) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Ala27-Arg1 91. It is sometimes possible for the material contained within the vial of "VEGF165/VEGFA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.