Standard Curve (Sample)
(Fig.1. Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) Standard Curve.)
Human Vascular Endothelial Cell Growth Factor Receptor 2 (VEGFR-2/Flk-1) ELISA Kit | VEGFR2 elisa kit
Human Vascular Endothelial Cell Growth Factor Receptor 2 (VEGFR-2/Flk-1) ELISA Kit
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human
Synonyms
Vascular Endothelial Cell Growth Factor Receptor 2 (VEGFR-2/Flk-1); N/A; Human Vascular Endothelial Cell Growth Factor Receptor 2 (VEGFR-2/Flk-1) ELISA Kit; KDR; CD309; FLK1; VEGFR; fetal liver kinase-1; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; vascular endothelial growt; VEGFR2 elisa kit
Reactivity
Human
Specificity
Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KDR. No significant cross-reactivity or interference between Human KDR and analogues was observed.
Sequence Length
1356
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) Standard Curve.)
Related Product Information for VEGFR2 elisa kit
Description: This Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit employs a two-site sandwich ELISA to quantitate KDR in samples. An antibody specific for KDR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KDR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of KDR are implicated in infantile capillary hemangiomas.
Background: Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of KDR are implicated in infantile capillary hemangiomas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vascular endothelial growth factor receptor 2
NCBI Official Synonym Full Names
kinase insert domain receptor
NCBI Official Symbol
KDR
NCBI Official Synonym Symbols
FLK1; CD309; VEGFR; VEGFR2
NCBI Protein Information
vascular endothelial growth factor receptor 2
UniProt Protein Name
Vascular endothelial growth factor receptor 2
UniProt Gene Name
KDR
UniProt Synonym Gene Names
FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
UniProt Entry Name
VGFR2_HUMAN
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Product Notes
The Human VEGFR2 kdr (Catalog #AAA331949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331949 ELISA Kit recognizes Human VEGFR2. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Cell Growth Factor Receptor 2 (VEGFR-2/Flk-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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