Standard Curve (Sample)
Human Brorin ELISA Kit | VWC2 elisa kit
Human Brorin PicoKine ELISA Kit
Gene Names
VWC2; UNQ739; PSST739
Reactivity
Human
Synonyms
Brorin; N/A; Human Brorin PicoKine ELISA Kit; Human Brorin; von Willebrand factor C domain containing 2; Brain-specific chordin-like protein; von Willebrand factor C domain-containing protein 2; VWC2; UNQ739; PRO1434; VWC2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human VWC2
Sequence Length
325
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for VWC2 elisa kit
Background: Von Willebrand factor, type C (VWFC or VWC)is a protein domain is found in various blood plasma proteins: complement factors B, C2, CR3 and CR4; the integrins (I-domains); collagen types VI, VII, XII and XIV; and other extracellular proteins. It is mapped to 7p12.2. This gene encodes a secreted bone morphogenic protein antagonist. The encoded protein is possibly involved in neural function and development and may have a role in cell adhesion.
Principle of the Assay: The Picokine™ Human VWC2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human VWC2 with a 96-well strip plate that is pre-coated with antibody specific for VWC2. The detection antibody is a biotinylated antibody specific for VWC2. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human VWC2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S28-M325. The kit is analytically validated with ready-to-use reagents. To measure Human VWC2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human VWC2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human VWC2 in the sample.
Principle of the Assay: The Picokine™ Human VWC2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human VWC2 with a 96-well strip plate that is pre-coated with antibody specific for VWC2. The detection antibody is a biotinylated antibody specific for VWC2. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human VWC2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S28-M325. The kit is analytically validated with ready-to-use reagents. To measure Human VWC2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human VWC2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human VWC2 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Brorin
NCBI Official Synonym Full Names
von Willebrand factor C domain containing 2
NCBI Official Symbol
VWC2
NCBI Official Synonym Symbols
UNQ739; PSST739
NCBI Protein Information
brorin
UniProt Protein Name
Brorin
UniProt Gene Name
VWC2
UniProt Entry Name
VWC2_HUMAN
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Product Notes
The Human VWC2 vwc2 (Catalog #AAA125197) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125197 ELISA Kit recognizes Human VWC2. It is sometimes possible for the material contained within the vial of "Brorin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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