ELISA
(ELISA for XRCC1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of XRCC1 were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
XRCC1 recombinant protein
Recombinant XRCC1 protein
Purity
>=80% by SDS-PAGE.
Synonyms
XRCC1; N/A; Recombinant XRCC1 protein; RCC; SCAR26; XRCC1 recombinant protein
Host
Baculovirus
Purity/Purification
>=80% by SDS-PAGE.
Form/Format
Recombinant XRCC1 protein is supplied at in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant XRCC1 protein that includes full length of human XRCC1 protein (accession number NP_006288.2) was expressed in Sf9 cells and contains an C-terminal His-DYKDDDDK tag with a molecular weight of 72.01kDa.
ELISA
(ELISA for XRCC1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of XRCC1 were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA
(ELISA for XRCC1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of XRCC1 were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
SDS-PAGE
(Recombinant XRCC1 protein gel. XRCC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 72.01kDa Purity: >80%)
SDS-PAGE
(Recombinant XRCC1 protein gel. XRCC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 72.01kDa Purity: >80%)
Related Product Information for XRCC1 recombinant protein
XRCC1 (DNA repair protein XRCC1) also known as RCC and SCAR26. XRCC1 is a scaffold protein that is recruited to SSBs by PARP1 and/or PARP2 activity through direct interaction betweenpoly (ADP-ribose) and the central BRCT domain in XRCC1. XRCC1 in turn interacts with and recruits the enzymes required for SSB repair, including DNA polymerase b, DNA ligase III, polynucleotide kinase/phosphatase and aprataxin. Hereditarymutations in XRCC1 and some of these protein partners result inneurodevelopmental disorders and/or progressive neurodegeneration.
Product Categories/Family for XRCC1 recombinant protein
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Product Notes
The XRCC1 (Catalog #AAA60405) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "XRCC1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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