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FCM/FACS (Flow Cytometry)
(XRCC1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human XRCC1 Polyclonal Antibody | anti-XRCC1 antibody
XRCC1 Antibody (Center)
Gene Names
XRCC1; RCC
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
XRCC1, Antibody; XRCC1 Antibody (Center); DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; XRCC1; anti-XRCC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This XRCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human XRCC1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
407-435
Sequence Length
633
Applicable Applications for anti-XRCC1 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(XRCC1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(XRCC1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(XRCC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(XRCC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(XRCC1 Antibody (Center) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow).)
WB (Western Blot)
(XRCC1 Antibody (Center) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow).)
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells, using XRCC1 Antibody (Center). AAA287156 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells, using XRCC1 Antibody (Center). AAA287156 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Related Product Information for anti-XRCC1 antibody
The protein encoded by this gene is involved in the
efficient repair of DNA single-strand breaks formed by exposure to
ionizing radiation and alkylating agents. This protein interacts
with DNA ligase III, polymerase beta and poly (ADP-ribose)
polymerase to participate in the base excision repair pathway. It
may play a role in DNA processing during meiogenesis and
recombination in germ cells. A rare microsatellite polymorphism in
this gene is associated with cancer in patients of varying
radiosensitivity.
efficient repair of DNA single-strand breaks formed by exposure to
ionizing radiation and alkylating agents. This protein interacts
with DNA ligase III, polymerase beta and poly (ADP-ribose)
polymerase to participate in the base excision repair pathway. It
may play a role in DNA processing during meiogenesis and
recombination in germ cells. A rare microsatellite polymorphism in
this gene is associated with cancer in patients of varying
radiosensitivity.
Product Categories/Family for anti-XRCC1 antibody
References
Lamerdin, J.E., et al. Genomics 25(2):547-554(1995)
Gyapay, G., et al. Nat. Genet. 7 (2 SPEC NO), 246-339 (1994) :
Thompson, L.H., et al. Mol. Cell. Biol. 10(12):6160-6171(1990)
Thompson, L.H., et al. Genomics 5(4):670-679(1989)
Gyapay, G., et al. Nat. Genet. 7 (2 SPEC NO), 246-339 (1994) :
Thompson, L.H., et al. Mol. Cell. Biol. 10(12):6160-6171(1990)
Thompson, L.H., et al. Genomics 5(4):670-679(1989)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69477
NCBI Official Full Name
DNA repair protein XRCC1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol
XRCC1
NCBI Official Synonym Symbols
RCC
NCBI Protein Information
DNA repair protein XRCC1
UniProt Protein Name
DNA repair protein XRCC1
UniProt Gene Name
XRCC1
UniProt Entry Name
XRCC1_HUMAN
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Product Notes
The XRCC1 xrcc1 (Catalog #AAA287156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 407-435. The XRCC1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XRCC1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the XRCC1 xrcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XRCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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