Application Data
(SARS-CoV-2 Spike protein RBD his/sumostar tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 100 nM as )
Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S) Active Protein
Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Synonyms
Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S); N/A; Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active); Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S) active protein
Host
Yeast
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Sequence Positions
319-541aa; Partial
Sequence
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF$
Species
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)(SARS-CoV-2)
Tag
N-terminal 6xHis-sumostar-tagged
Endotoxin
Not test.
Research Area
Microbiology
Biological Activity
1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind SARS-CoV-2-S Antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 ug/ml can bind human ACE2, the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind SARS-CoV-2-S Antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.?SARS-CoV-2 Spike protein RBD his/sumostar tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 100 nM as detected by LSPR Assay.?SARS-CoV-2 Spike protein RBD His/Sumostar Tag captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody with an affinity constant of 28.2nM as detected by LSPR Assay.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Application Data
(SARS-CoV-2 Spike protein RBD his/sumostar tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 100 nM as )
Application Data
(SARS-CoV-2 Spike protein RBD his/sumostar tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 100 nM as )
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ?g/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.)
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ?g/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.Predicted band size: 38.2 kDaObserved band size:(a) 68 kDa before EndoH Digestion(b) 38 kDa after EndoH Digestion)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.Predicted band size: 38.2 kDaObserved band size:(a) 68 kDa before EndoH Digestion(b) 38 kDa after EndoH Digestion)
Product Categories/Family for Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S) active protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
38.2kDa
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Product Notes
The Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S) (Catalog #AAA279233) is an Active Protein produced from Yeast and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-541aa; Partial. The amino acid sequence is listed below: RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF$. It is sometimes possible for the material contained within the vial of "Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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