Standard Curve (Sample)
Mouse Complement C3 ELISA Kit | C3 elisa kit
Mouse Complement C3 ELISA kit
Gene Names
C3; ASP; Plp; HSE-MSF; AI255234
Reactivity
Mouse
Synonyms
Complement C3; Mouse Complement C3 ELISA kit; C3 elisa kit
Reactivity
Mouse
Specificity
This assay recognizes natural mouse C3. Pooled normal plasma from human, pig, rabbit, sheep, canine, and horse were assayed and no significant crossreactivity was observed. Pooled normal plasma from rat and rabbit resulted in significant color development.
Form/Format
Complete kit
Sequence Length
1663
Samples
Mouse plasma, serum, and ce ll culture samples
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concen tration were tested in ten independent assays to assess inter-assay precision.
Preparation and Storage
Recommended Storage: 4 degree C
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Shipping Condition: Cold pack
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Shipping Condition: Cold pack
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for C3 elisa kit
Background: Complement C3, the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation [1]. C3 is proteolytical ly activated by C3- convertase to the anaphylatoxin C3a and the opsonizing agent C3b [2 ]. Serum concentrations of C3 are increased during acute and chronic inflammation such as rheumatoid arthritis, and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus [3].
Principle of the Assay: Mouse C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled polyclonal anti-mouse C3 antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 4S0nm. A standard cal ibration curve is prepared along with the samples to be measured using dilutions of mouse C3. Color development is proportional to the concentration of C3 in the samples.
Intended Uses: This mouse complement component 3 (01 total antigen assay is intended for the quantitative determination of total C3 antigen in mouse plasma, serum, and cell culture samples. For research use only,
Principle of the Assay: Mouse C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled polyclonal anti-mouse C3 antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 4S0nm. A standard cal ibration curve is prepared along with the samples to be measured using dilutions of mouse C3. Color development is proportional to the concentration of C3 in the samples.
Intended Uses: This mouse complement component 3 (01 total antigen assay is intended for the quantitative determination of total C3 antigen in mouse plasma, serum, and cell culture samples. For research use only,
Product Categories/Family for C3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,952 Da
NCBI Official Full Name
complement C3
NCBI Official Synonym Full Names
complement component 3
NCBI Official Symbol
C3
NCBI Official Synonym Symbols
ASP; Plp; HSE-MSF; AI255234
NCBI Protein Information
complement C3; complement factor 3; complement component 3d; acylation stimulating protein
UniProt Protein Name
Complement C3
UniProt Gene Name
C3
UniProt Synonym Gene Names
C3bc; ASP
UniProt Entry Name
CO3_MOUSE
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Product Notes
The Mouse C3 c3 (Catalog #AAA10799) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA10799 ELISA Kit recognizes Mouse C3. It is sometimes possible for the material contained within the vial of "Complement C3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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