Mouse IgG Monoclonal Antibody | anti-IgG antibody
MOUSE ANTI HUMAN IgG (Fc) (CH2 DOMAIN)
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Purified IgG - liquid
Synonyms
IgG; Monoclonal Antibody; MOUSE ANTI HUMAN IgG (Fc) (CH2 DOMAIN); anti-IgG antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8A4
Specificity
IgG (Fc) (CH2 DOMAIN)
Mouse anti Human IgG (Fc) (CH2 Domain) antibody, clone 8A4 recognizes an eptitope within the CH2 domain of the Fc region of all sebclasses of human IgG.
Mouse anti Human IgG (Fc) (CH2 Domain) antibody, clone 8A4 recognizes an eptitope within the CH2 domain of the Fc region of all sebclasses of human IgG.
Purity/Purification
Purified IgG - liquid
Form/Format
Phosphate buffered saline
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
377
Applicable Applications for anti-IgG antibody
Immunohistology Frozen (IH-F), ELISA (EIA), Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatan
Carrier Free
Yes
Immunogen
Human IgG polyclonal
Fusion Partners
Spleen cells from immunised Balb/c mouse were fused with cells of the P3-NS1/Ag1 myeloma cell line.
Target Species
Human
Species Cross-Reactivity
Reacts with: Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within laboratories , peer reviewed publications or personal comunications from the origanators.
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within laboratories , peer reviewed publications or personal comunications from the origanators.
Preparation and Storage
It is recommended to aliquot and store at -20 degrees C on receipt.
When thawed, aliquot the sample as needed Keep aliquots at 2-8 degrees C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degrees C. Avoid freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended
When thawed, aliquot the sample as needed Keep aliquots at 2-8 degrees C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degrees C. Avoid freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended
Related Product Information for anti-IgG antibody
Mouse anti Human IgG (Fc) (CH2 Domain) antibody, clone 8A4 recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human IgG.
Similar Products
Product Notes
The IgG (Catalog #AAA11963) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen (IH-F), ELISA (EIA), Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100 Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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