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Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:RPE)
Rat CD83 Monoclonal Antibody | anti-CD83 antibody
RAT ANTI MOUSE CD83
Applications
Flow Cytometry, Functional Assay
Synonyms
CD83; Monoclonal Antibody; RAT ANTI MOUSE CD83; anti-CD83 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3D11
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
196
Applicable Applications for anti-CD83 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Fusion Partners
Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 mouse myeloma cell line.
Buffer Solution
Buffer Solution
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from the date of despatch.
Shelf Life: 18 months from the date of despatch.
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:RPE)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:RPE)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:Alexa Fluor 647)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:Alexa Fluor 647)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:FITC)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:FITC)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:Alexa Fluor 488)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:Alexa Fluor 488)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83)
Application Data
(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83)
Related Product Information for anti-CD83 antibody
Rat anti Mouse CD83 antibody, clone 3D11 recognizes murine CD83, a 45kD type 1 glyoprotein belonging to the immunoglobulin superfamily. Originally described as a maturation marker for dendritic cells, CD83 plays a role in the regulation of antigen presentation, thymic T-cell maturation, B-cell function and peripheral T-cell activation. Activated dendritic cells and B-cells release a soluble form of CD83 that is reported to have an immunosuppressive effect, suggesting a therapeutic role for sCD83 in the regulation of immune disorders.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,313 Da
NCBI Official Full Name
CD83 antigen isoform 1
NCBI Official Synonym Full Names
CD83 antigen
NCBI Official Symbol
Cd83
NCBI Protein Information
CD83 antigen; mCD83
UniProt Protein Name
CD83 antigen
UniProt Gene Name
Cd83
UniProt Synonym Gene Names
mCD83
UniProt Entry Name
CD83_MOUSE
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Product Notes
The CD83 cd83 (Catalog #AAA12212) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD83 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD83 cd83 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD83, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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