stem cell factor Active Protein | mSCF active protein
Mouse stem cell factor (mSCF)
Gene Names
KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
Purity
98.0% as determined by:(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(d) Gel filtration chromatography under non denaturing conditions.
Synonyms
stem cell factor; Mouse stem cell factor (mSCF); mSCF active protein
Host
E Coli
Purity/Purification
98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(d) Gel filtration chromatography under non denaturing conditions.
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(d) Gel filtration chromatography under non denaturing conditions.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Ile-Cys.
Sequence Length
37
Solubility
It is recommended to reconstitute the lyophilized rHuSCF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions conteining buffers and carrier proteins.
Protein Content
Protein content:Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
Less than 2% as determined by gel filtration chromatography under non denaturing conditions.
Endotoxin
Less than 0.02 ng/ug (IEU/ug) of mSCF.
Formulation
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.
Biological Activity
mSCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of 105 IU/mg.
Preparation and Storage
Lyophilized rmSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution rHuSCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Related Product Information for mSCF active protein
Recombinant murine SCF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18309 Dalton. Bioactive mouse SCF appears as a non covalent homodimer (Lu et al. (1991) JBC, 266, 8102-8107).
Product Categories/Family for mSCF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,667 Da
NCBI Official Full Name
stem cell factor, partial
NCBI Official Synonym Full Names
KIT ligand
NCBI Official Symbol
KITLG
NCBI Official Synonym Symbols
SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
NCBI Protein Information
kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor
UniProt Protein Name
Kit ligand
UniProt Gene Name
KITLG
UniProt Synonym Gene Names
MGF; SCF; MGF; SCF; sKITLG
UniProt Entry Name
SCF_HUMAN
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Product Notes
The mSCF kitlg (Catalog #AAA13622) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Gl u-Ile-Cys. It is sometimes possible for the material contained within the vial of "stem cell factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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