Mouse anti-Human CXCR3 Monoclonal Antibody | anti-CXCR3 antibody

Mouse anti CXCR3 Monoclonal antibody

Cat. Num. AAA14093

• Gene Names: CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Immunohistochemistry
• Purity: The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
• Synonyms: CXCR3; Monoclonal Antibody; Mouse anti CXCR3 Monoclonal antibody; Mouse anti-CXCR3; Chemokine CXC Motif receptor 3; CD183; GPR9; IP10R; MIGR; anti-CXCR3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 1C6
• Specificity: This antibody recognizes surface expressed CXCR3, and/or PHA or anti-CD3 activated human T cells. The other species are not tested.
• Purity/Purification: The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
• Form/Format: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-CXCR3 antibody: ELISA, Flow Cytometry, Immunohistochemistry
• Application Notes: Western Blot: 0.5-2 ug/ml; ELISA: 0.1-1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 1:500; Flow Cytometry: 2-5 ug/ml
• Antigen Preparation: A 37aa-synthetic peptide corresponding to the N-terminus of human CXCR3 conjugated to KLH.
• Positive Control: Transfected CXCR3 Cells
• Cellular Location: Cell Membrane
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Application Data

Related Product Information for anti-CXCR3 antibody

Chemokine receptors are seven-transmembrane domain G-protein coupled receptors that mediate the biological activities of chemokines. Most of these receptors exhibit promiscuous binding properties whereby several different chemokines signal through the same receptor. They are named according to the chemokine subfamily they bind. There are currently six CXCspecific receptors designated CXCR1 to CXCR6. This antibody recognizes surface expressed CXCR3, as judged by staining of CXCR3 transfected cells, but not untransfected cells. The antibody also stained human T cells and T cell clones that had been activated in vitro with PHA or anti-CD3. However, the anti-CXCR3 antibody did not react with neutrophils. The CXCR3 receptor is also known as the IP-10 receptor and the Mig receptor.

References

Qin S, et al. The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. J. Clin. Invest. 101: 746-754, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 218563730
• NCBI GeneID: 2833
• NCBI Accession #: NP_001136269.1
• NCBI GenBank Nucleotide #: NM_001142797.1
• UniProt Accession #: P49682; O15185; Q7Z710; Q9P2T4; Q9P2T5
• Molecular Weight: ~41 kDa
• NCBI Official Full Name: C-X-C chemokine receptor type 3 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 3
• NCBI Official Symbol: CXCR3
• NCBI Official Synonym Symbols: GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• NCBI Protein Information: C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; OTTHUMP00000032170; OTTHUMP00000070257; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor
• UniProt Protein Name: C-X-C chemokine receptor type 3
• UniProt Gene Name: CXCR3
• UniProt Synonym Gene Names: GPR9
• UniProt Entry Name: CXCR3_HUMAN