Application Data
Rabbit anti-Rat Interferon-a Receptor, Type I, Subunit I (Ser535,539) Polyclonal Antibody | anti-IFNAR1 antibody
Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I
Gene Names
IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Synonyms
Interferon-a Receptor; Type I; Subunit I (Ser535; 539); Polyclonal Antibody; Anti-Phospho-Ser535; 539 Interferon-alpha Receptor; Subunit I; anti-IFNAR1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 - 130k.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
Sequence Length
557
Applicable Applications for anti-IFNAR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:1000
WB: 1:1000
IHC: 1:1000
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of human interferon-alpha receptor, type I, subunit I (IFNAR1).
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH
Immunogen Species
Human
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, human, mouse, non-human primates and sheep
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primates and sheep based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-alpha Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Application Data
Application Data
Related Product Information for anti-IFNAR1 antibody
Affinity purified rabbit polyclonal antibody
References
• Biron CA (2001) Interferons alpha and beta as immune regulators--a new look. Immunity 14:661-664.
• Kirkwood J (2002) Cancer immunotherapy: the interferon-alpha experience. Semin Oncol 29:18-26.
• Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY (2003) SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor. EMBO J 22:5480-5490.
• K. G. Suresh Kumar, John J. Krolewski, and Serge Y. Fuchs (2004) Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 - 46620.
• Kirkwood J (2002) Cancer immunotherapy: the interferon-alpha experience. Semin Oncol 29:18-26.
• Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY (2003) SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor. EMBO J 22:5480-5490.
• K. G. Suresh Kumar, John J. Krolewski, and Serge Y. Fuchs (2004) Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 - 46620.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110-130
NCBI Official Full Name
interferon alpha/beta receptor 1
NCBI Official Synonym Full Names
interferon (alpha, beta and omega) receptor 1
NCBI Official Symbol
IFNAR1
NCBI Official Synonym Symbols
AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC
NCBI Protein Information
interferon alpha/beta receptor 1; CRF2-1; IFN-R-1; IFN-alpha/beta receptor 1; interferon-beta receptor 1; beta-type antiviral protein; alpha-type antiviral protein; type I interferon receptor 1; cytokine receptor class-II member 1; cytokine receptor family 2 member 1; interferon-alpha/beta receptor alpha chain
UniProt Protein Name
Interferon alpha/beta receptor 1
UniProt Gene Name
IFNAR1
UniProt Synonym Gene Names
IFNAR; IFN-R-1; IFN-alpha/beta receptor 1; CRF2-1
UniProt Entry Name
INAR1_HUMAN
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Product Notes
The IFNAR1 ifnar1 (Catalog #AAA14231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon-a Receptor, Type I, Subunit I (Ser535,539) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Quality Control: Western blots performed on each lot. WB: 1:1000 IHC: 1:1000. Researchers should empirically determine the suitability of the IFNAR1 ifnar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon-a Receptor, Type I, Subunit I (Ser535,539), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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