Mouse CD62L Monoclonal Antibody | anti-CD62L antibody

anti-human CD62L-APC

Cat. Num. AAA14858

• Reactivity: Human, Horse, Mouse, Guinea Pig, Rat, Dog, Cow
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD62L; Monoclonal Antibody; anti-human CD62L-APC; Monoclonal anti-human CD62L (L-selectin)/APC; anti-CD62L antibody

• Host: Mouse
• Reactivity: Human, Horse, Mouse, Guinea Pig, Rat, Dog, Cow
• Clonality: Monoclonal
• Isotype: IgG2ak
• Clone Number: LAM1-116
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Concentration: Volume: 0.2 ml (varies by lot)

• Applicable Applications for anti-CD62L antibody: Flow Cytometry (FC/FACS)
• Application Notes: 1:50 (or use 1.6ul of concentrated stock per 5 x 10⁵cell test)
• Conjugation: APC
• Production: Protein A purified antibody from tissue culture supernatant was conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A₆₅₀/A₂₈₀ ration of 2.21.
• Immunogen: Mouse pre-B cells expressing human L-selectin
• Performance: Five x 10^5 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD62L/APC at a 1:50 dilution factor (10ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.1 log ₁₀ fluorescent units when compared to a Mouse IgG2a/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD62L antibody.
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Application Data

Related Product Information for anti-CD62L antibody

Information: Human CD62L is as adhesion molecule found on most leukocytes and plays a primary role in mediating initial leukocyte interaction with activated vascular endothelium (1). Human CD62L has signal transduction functions where engagement leads to increased calcium levels and upregulated expression of certain integrins (2). Antibody LAM1-116 recognizes the lectin binding domain of CD62L (2). Antibody LAM1-116 blocks CD62L function and induces expression of beta-1 and beta-2 integrins (1).

References

(1). T.A. Springer, (1995) Annu Rev Physiol 57: 827-872. (2). D.A. Steeber, et al, (1997) J Immunol 159: 952-963. (3). Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 420-424.