Mouse Leukocyte antigen E ELISA Kit | HLA-E elisa kit

Mouse Leukocyte antigen E ELISA Kit

Cat. Num. AAA17178

• Gene Names: HLA-E; MHC; QA1; EA1.2; EA2.1; HLA-6.2
• Reactivity: Mouse
• Synonyms: Leukocyte antigen E; Mouse Leukocyte antigen E ELISA Kit; HLA-E elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HLAE. No significant cross-reactivity or interference between HLAE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HLAE and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Intended Uses: This HLA-E ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HLA-E. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This HLA-E ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HLA-E. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for HLA-E elisa kit

Principle of the Assay: HLAE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HLAE antibody and an HLAE-HRP conjugate. The assay sample and buffer are incubated together with HLAE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HLAE concentration since HLAE from samples and HLAE-HRP conjugate compete for the anti-HLAE antibody binding site. Since the number of sites is limited, as more sites are occupied by HLAE from the sample, fewer sites are left to bind HLAE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HLAE concentration in each sample is interpolated from this standard curve.

Intended Uses: This HLAE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HLAE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for HLA-E elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 62912479
• NCBI GeneID: 3133
• NCBI Accession #: NP_005507.3
• NCBI GenBank Nucleotide #: NM_005516.5
• Molecular Weight: 40,157 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain E
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, E
• NCBI Official Symbol: HLA-E
• NCBI Official Synonym Symbols: MHC; QA1; EA1.2; EA2.1; HLA-6.2
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain E; MHC HLA-E alpha-1; lymphocyte antigen; MHC HLA-E alpha-2.1; MHC class I antigen E; HLA class I histocompatibility antigen, E alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain E
• UniProt Gene Name: HLA-E
• UniProt Synonym Gene Names: HLA-6.2; HLAE
• UniProt Entry Name: HLAE_HUMAN