E-Cadherin Recombinant Antibody | CDH1 recombinant antibody

Rabbit anti-E-Cadherin Recombinant Monoclonal Antibody [BLR088G]

Cat. Num. AAA23828

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: E-Cadherin, Recombinant Antibody; Rabbit anti-E-Cadherin Recombinant Monoclonal Antibody [BLR088G]; LCAM; CDHE; UVO; epithelial cadherin; epididymis secretory sperm binding protein; E-cadherin 1; E-cadherin; ECAD; cell-CAM 120/80; CD antigen CD324; CAM 120/80; calcium-dependent adhesion protein, epithelial; cadherin-1; cadherin 1, type 1, E-cadherin (epithelial); cadherin 1, E-cadherin (epithelial); Arc-1; BCDS1; uvomorulin; CD324; Cadherin-1; cadherin 1; CDH1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR088G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 250ug/ml (varies by lot)

• Applicable Applications for CDH1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.1 ul per 1 x 10^6 cells.
• Immunogen: Residues 155-707 (ECD)
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human E-Cadherin by western blot. Samples: Whole cell lysate (5 ug) from GaMG, HCT 116, U2OS, OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of mouse E-Cadherin by western blot. Samples: Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3, Renca, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation)

(Detection of human E-Cadherin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1) used for IP at 20 ul/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody (BL13080). For blotting immunoprecipitated E-Cadherin, AAA23828 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistchemistry)

(Detection of human E-Cadherin in FFPE lung carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)

IHC (Immunohistochemistry)

(Detection of mouse E-Cadherin in FFPE skin by IHC. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human E-Cadherin in FFPE squamous cell carcinoma by IHC. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human E-Cadherin in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human E-Cadherin in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (AAA23828) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for CDH1 recombinant antibody

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in adjacent cells. E-Cadherin, or Cadherin-1, is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P12830].

Product Categories/Family for CDH1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4757960
• NCBI GeneID: 999
• NCBI Accession #: NP_004351.1
• NCBI GenBank Nucleotide #: NM_004360.3
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; A8K1U7
• Molecular Weight: 97,456 Da
• NCBI Official Full Name: cadherin-1 preproprotein
• NCBI Official Synonym Full Names: cadherin 1, type 1, E-cadherin (epithelial)
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• NCBI Protein Information: cadherin-1; CAM 120/80; E-Cadherin; uvomorulin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial
• UniProt Protein Name: Cadherin-1
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin
• UniProt Entry Name: CADH1_HUMAN