Standard Curve (Sample)
Human Interleukin 8 ELISA Kit | IL-8 elisa kit
Human Interleukin 8 ELISA Kit
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Reactivity
Human
Synonyms
Interleukin 8; Human Interleukin 8 ELISA Kit; IL-8/IL8/CXCL8/GCP-1/GCP1/LECT/LUCT/LYNAP/MDNCF/MONAP/NAF/NAP-1/NAP1/3-10C/AMCF-I/K60; IL-8 elisa kit
Reactivity
Human
Specificity
Specifically recognize IL-8, no obvious cross reaction with other analogues
Sequence Length
99
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-8 elisa kit
Background: Interleukin-8 (IL-8) is a cytokine belonging to the chemokine family, mainly produced by macrophages, endothelial cells, and epithelial cells after stimulation by inflammation, pathogen infection, and other factors. IL-8 can induce white blood cells to migrate to the site of inflammation through its receptors CXCR1 and CXCR2, and participate in physiological and pathological processes such as inflammatory response, immune response, and tumor occurrence. In addition, IL-8 also has functions such as promoting angiogenesis, cell proliferation, and metastasis, and is therefore considered a cytokine associated with tumors. IL-8 plays an important role in the development of various diseases such as cancer, cardiovascular disease, and autoimmune diseases.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IL-8 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti IL-8 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with IL-8 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IL-8 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IL-8 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti IL-8 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with IL-8 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IL-8 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,098 Da
NCBI Official Full Name
interleukin-8
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
NCBI Protein Information
interleukin-8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
CXCL8
UniProt Synonym Gene Names
IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF
UniProt Entry Name
IL8_HUMAN
Similar Products
Product Notes
The Human IL-8 cxcl8 (Catalog #AAA17520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17520 ELISA Kit recognizes Human IL-8. It is sometimes possible for the material contained within the vial of "Interleukin 8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
