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WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)
Rabbit Cdk2/Cdc2 Polyclonal Antibody | anti-Cdk2/Cdc2 antibody
Cdk2/Cdc2 (Phospho-Thr160) Rabbit Polyclonal Antibody
Gene Names
CDK2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Cdk2/Cdc2; Polyclonal Antibody; Cdk2/Cdc2 (Phospho-Thr160) Rabbit Polyclonal Antibody; CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; BH6842; anti-Cdk2/Cdc2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody detects endogenous levels of Cdk2/Cdc2 protein only when phosphorylated at T160.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Cdk2/Cdc2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/20000
IHC: 1/100-1/300
ELISA: 1/20000
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Cdk2/Cdc2 (phospho Thr160)
Modification
Phospho Specific
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
ELISA
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK2/CDC2 (Phospho-Thr160) Antibody)
ELISA
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK2/CDC2 (Phospho-Thr160) Antibody)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using CDK2/CDC2 (Phospho-Thr160) Antibody. The picture on the right is blocked with the CDK2/CDC2 (Phospho-Thr160) peptide.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using CDK2/CDC2 (Phospho-Thr160) Antibody. The picture on the right is blocked with the CDK2/CDC2 (Phospho-Thr160) peptide.)
Related Product Information for anti-Cdk2/Cdc2 antibody
cyclin dependent kinase 2 (CDK2) Homo sapiens This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,930 Da
NCBI Official Full Name
cyclin-dependent kinase 2 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 2
NCBI Official Symbol
CDK2
NCBI Official Synonym Symbols
p33(CDK2)
NCBI Protein Information
cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2
UniProt Protein Name
Cyclin-dependent kinase 2
UniProt Gene Name
CDK2
UniProt Synonym Gene Names
CDKN2
UniProt Entry Name
CDK2_HUMAN
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Product Notes
The Cdk2/Cdc2 cdk2 (Catalog #AAA30744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cdk2/Cdc2 (Phospho-Thr160) Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cdk2/Cdc2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC: 1/100-1/300 ELISA: 1/20000. Researchers should empirically determine the suitability of the Cdk2/Cdc2 cdk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdk2/Cdc2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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