Standard Curve (Sample)
Human Immunoglobulin J chain ELISA Kit | IGJ elisa kit
Human Immunoglobulin J chain ELISA Kit
Gene Names
JCHAIN; IGJ; JCH; IGCJ
Reactivity
Human
Synonyms
Immunoglobulin J chain; Human Immunoglobulin J chain ELISA Kit; IGJ/Immunoglobulin J chain/IGCJ; IGJ elisa kit
Reactivity
Human
Specificity
Specifically recognize IGJ, no obvious cross reaction with other analogues
Sequence Length
159
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IGJ elisa kit
Background: The immunoglobulin J chain (Ig J chain) is a linker protein for two monomer units of either immunoglobulin alpha (IgA) or mu (IgM) polypeptides. For IgA the J chained-joined dimer induces larger polymers whereas for the IgM pentamer it functions as a nucleating unit. The Ig J-chain is also important in binding these immunoglobulins to secretory components.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IGJ antibody was pre-coated onto the 96-well plate. The HRP conjugated anti IGJ antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, HRP detection antibody was added to bind with IGJ conjugated on coated antibody. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IGJ in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IGJ antibody was pre-coated onto the 96-well plate. The HRP conjugated anti IGJ antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, HRP detection antibody was added to bind with IGJ conjugated on coated antibody. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IGJ in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,099 Da
NCBI Official Full Name
immunoglobulin J chain
NCBI Official Synonym Full Names
joining chain of multimeric IgA and IgM
NCBI Official Symbol
JCHAIN
NCBI Official Synonym Symbols
IGJ; JCH; IGCJ
NCBI Protein Information
immunoglobulin J chain
UniProt Protein Name
Immunoglobulin J chain
UniProt Gene Name
JCHAIN
UniProt Entry Name
IGJ_HUMAN
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Product Notes
The Human IGJ jchain (Catalog #AAA17654) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17654 ELISA Kit recognizes Human IGJ. It is sometimes possible for the material contained within the vial of "Immunoglobulin J chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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