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IHC (Immunohistochemistry)
(MDH/MDH2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
Goat anti-Human MDH/MDH2 Polyclonal Antibody | anti-MDH2 antibody
MDH/MDH2 Goat anti-Human Polyclonal (aa221-232) Antibody
Gene Names
MDH2; MDH; MOR1; M-MDH; MGC:3559
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MDH/MDH2; Polyclonal Antibody; MDH/MDH2 Goat anti-Human Polyclonal (aa221-232) Antibody; IHC-plus MDH/MDH2 Antibody (aa221-232); MDH2; MDH; M-MDH; MGC:3559; MOR1; anti-MDH2 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
TBS, pH7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-MDH2 antibody
Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)
Application Notes
IHC-P: 5ug/ml
Peptide-ELISA: 1:16000
WB: 0.01-0.03ug/ml
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates (calculated MW of 35.5kDa according to NP_005909.2). Recommended concentration: 0.01-0.03ug/ml.
Peptide-ELISA: 1:16000
WB: 0.01-0.03ug/ml
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates (calculated MW of 35.5kDa according to NP_005909.2). Recommended concentration: 0.01-0.03ug/ml.
Target
Human MDH/MDH2
Immunogen
Peptide with sequence C-DQLTALTGRIQE, from the internal region of the protein sequence according to NP_005909.2.
Conjugation
Unconjugated
Epitope
aa221-232
Preparation and Storage
Aliquot and store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(MDH/MDH2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(MDH/MDH2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(MDH/MDH2 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(MDH/MDH2 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(MDH/MDH2 Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(MDH/MDH2 Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(MDH/MDH2 Antibody-Antibody (0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Antibody (0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Antibody (0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Antibody (0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Goat anti-MDH2 (aa221-232) Antibody (0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Goat anti-MDH2 (aa221-232) Antibody (0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Goat anti-MDH2 (aa221-232) Antibody (0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)
WB (Western Blot)
(MDH/MDH2 Antibody-Goat anti-MDH2 (aa221-232) Antibody (0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)
Related Product Information for anti-MDH2 antibody
MDH2 antibody is an unconjugated goat polyclonal antibody to human MDH2 (MDH)(aa221-232). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
malate dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
malate dehydrogenase 2, NAD (mitochondrial)
NCBI Official Symbol
MDH2
NCBI Official Synonym Symbols
MDH; MOR1; M-MDH; MGC:3559
NCBI Protein Information
malate dehydrogenase, mitochondrial
UniProt Protein Name
Malate dehydrogenase, mitochondrial
UniProt Gene Name
MDH2
UniProt Entry Name
MDHM_HUMAN
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Product Notes
The MDH2 mdh2 (Catalog #AAA21369) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The MDH/MDH2 Goat anti-Human Polyclonal (aa221-232) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MDH/MDH2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB). IHC-P: 5ug/ml Peptide-ELISA: 1:16000 WB: 0.01-0.03ug/ml Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates (calculated MW of 35.5kDa according to NP_005909.2). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the MDH2 mdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDH/MDH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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