General Melatonin (MT) ELISA Kit | MT elisa kit
General Melatonin (MT) ELISA Kit
Reactivity
General
Synonyms
Melatonin (MT); N/A; General Melatonin (MT) ELISA Kit; N-Acetyl-5-Methoxytryptamine; MT elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of MT. No significant cross-reactivity or interference between MT and analogues was observed.
Assay Type
Quantitative Competitive
Samples
Serum, plasma and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
< 4.53pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for MT elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to MT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MT and unlabeled MT (Standards or samples) with the pre-coated antibody specific to MT. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MT in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MT in serum, plasma and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MT in serum, plasma and other biological fluids.
References
Per2 Expression Regulates the Spatial Working Memory of Mice through DRD1-PKA-CREB Signaling; Mol Neurobiol; 2022 Jul;59(7):4292-4303.; PMID: 35508866; Mikyung Kim; Uimyung Research Institute for Neuroscience, Department of Pharmacy, Sahmyook University, Hwarangro 815, Nowongu, Seoul, 01795, Republic of Korea. mkkim@syu.ac.kr.
