Mouse anti-Human CD74 Monoclonal Antibody | anti-CD74 antibody

Mouse anti-CD74 Monoclonal Antibody, Purified [LN2]

Cat. Num. AAA23781

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Purified
• Synonyms: CD74; Monoclonal Antibody; Mouse anti-CD74 Monoclonal Antibody; Purified [LN2]; HLADG; MHC HLA-DR gamma chain; II; Ia-GAMMA; Ia-associated invariant chain; Ia antigen-associated invariant chain; p33; HLA class II histocompatibility antigen gamma chain; gamma chain of class II antigens; DHLAG; CD74 molecule; major histocompatibility complex; class II invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex; class II antigen-associated); CD antigen CD74; HLA-DR-gamma; HLA-DR antigens-associated invariant chain; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: LN2
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-CD74 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Immunogen: Human SU-DHL-4 lymphoma cells
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD74 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Raji, GaMG, HeLa, SR, Jurkat, Daudi, and KG-1 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD74 monoclonal antibody [LN2] (AAA23781 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-RPS3 .)

IHC (Immunohistochemistry)

(Detection of human CD74 in FFPE colon carcinoma by IHC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human CD74 in FFPE lymph node by IHC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CD74 in FFPE Raji cells by ICC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CD74 in FFPE Daudi cells by ICC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human CD74 (shaded) in Ramos cells by flow cytometry. Antibody: Mouse anti-CD74 recombinant monoclonal [LN2] (AAA23781) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)

Related Product Information for anti-CD74 antibody

CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 also serves as cell surface receptor for the cytokine MIF. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P04233].

Product Categories/Family for anti-CD74 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 10835071
• NCBI GeneID: 972
• NCBI Accession #: NP_004346.1
• NCBI GenBank Nucleotide #: NM_004355.3
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4
• Molecular Weight: 18,328 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform b
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histo
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN