CD3E recombinant antibody

Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12]

Cat. Num. AAA23793

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified
• Synonyms: CD3E; Monoclonal Recombinant Antibody; Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12]; CD3-epsilon; TCRE; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex; epsilon subunit of T3; CD antigen CD3e; IMD18; CD3e molecule; epsilon (CD3-TCR complex); CD3e antigen; epsilon polypeptide (TiT3 complex); T3E; CD3 epsilon subunit of T-cell receptor complex; CD3E recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: BL-298-5D12
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 50ug/ml (varies by lot)

• Applicable Applications for CD3E recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.; mIF: 1:250
• Immunogen: Between 157 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD3E by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, K-562, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation)

(Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 2) used for IP at 20 ul/mg lysate. CD3E was also immunoprecipitated by a previous lot of this antibody (lot AAA23793-1). For blotting immunoprecipitated CD3E, AAA23793 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of human CD3E by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human CD3E (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry)

(Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (AAA23793 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human CD3E in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (AAA23793 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human CD3E by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for CD3E recombinant antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma,-delta and-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The epsilon polypeptide plays an essential role in T-cell development [taken from NCBI Entrez Gene (Gene ID: 916)].

Product Categories/Family for CD3E recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN