N-Cadherin Recombinant Antibody | CDH2 recombinant antibody

Rabbit anti-N-Cadherin Recombinant Monoclonal Antibody [BLR097G]

Cat. Num. AAA23831

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: N-Cadherin, Recombinant Antibody; Rabbit anti-N-Cadherin Recombinant Monoclonal Antibody [BLR097G]; cadherin-2; N-cadherin 1; N-cadherin; NCAD; CDw325; CDHN; CD325; calcium-dependent adhesion protein, neuronal; neural cadherin; cadherin 2, type 1, N-cadherin (neuronal); CD antigen CD325; Cadherin-2; cadherin 2; CDH2 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR097G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 500ug/ml (varies by lot)

• Applicable Applications for CDH2 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 12 ul/mg lysate; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Live and cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Residues 160-724 (ECD)
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse N-Cadherin by western blot. Samples: Whole cell lysate (50 ug) from C2C12, C2C12 treated with IFNgamma, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-N-Cadherin recombinant monoclonal antibody (AAA23831 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human N-Cadherin by western blot. Samples: Whole cell lysate (50 ug) from A-172, LNCaP, GaMG, Jurkat, OVCAR-8, RKO, Hep-G2, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-N-Cadherin recombinant monoclonal antibody (AAA23831 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human N-Cadherin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-N-Cadherin recombinant monoclonal antibody (AAA23831 lot 1) used for IP at 12 ul per reaction. N-Cadherin was also immunoprecipitated by rabbit anti-N-Cadherin antibody BL-1224-1D6. For blotting immunoprecipitated N-Cadherin, AAA23831 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

ICC (Immunocytochemistry)

(Detection of human N-Cadherin in FFPE NCI-H226 cells by ICC. Antibody: Rabbit anti-N-Cadherin recombinant monoclonal antibody (AAA23831 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human N-Cadherin in FFPE A-172 cells by ICC. Antibody: Rabbit anti-N-Cadherin recombinant monoclonal antibody (AAA23831 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human N-Cadherin (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-N-Cadherin recombinant monoclonal antibody (AAA23831) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for CDH2 recombinant antibody

N-Cadherin, or Cadherin-2, is a classical cadherin and member of the cadherin superfamily. N-Cadherin is a calcium-dependent cell adhesion molecule that plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [taken from NCBI Entrez Gene (Gene ID: 1000)].

Product Categories/Family for CDH2 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 14589889
• NCBI GeneID: 1000
• NCBI Accession #: NP_001783.2
• NCBI GenBank Nucleotide #: NM_001792.3
• UniProt Accession #: P19022; Q14923; Q8N173; B0YIY6
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin
• UniProt Entry Name: CADH2_HUMAN