SDS-PAGE
(SDS-PAGE Analysis Purified EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R). Confirmation of Integrity and Purity of Antibody.)
Rabbit EGFRvIII (Epidermal Growth Factor Receptor, Variant III) Monoclonal Antibody | anti-EGFR antibody
EGFRvIII (Epidermal Growth Factor Receptor, Variant III)
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
EGFRvIII (Epidermal Growth Factor Receptor, Variant III), Antibody; EGFRvIII (Epidermal Growth Factor Receptor, Variant III); Erbb1 variant III; ERBB1 variant III; HER1 variant III; Proto-oncogene c-ErbB-1 variant III; Receptor Tyrosine Protein Kinase variant III; ErbB1 variant III; anti-EGFR antibody
Host
Rabbit
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG
Clone Number
GFR/2600R
Specificity
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
Sequence Length
405
Applicable Applications for anti-EGFR antibody
Immunohistology (Formalin-Fixed)
Application Notes
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)
Positive Control
A431 Cells. Placenta, Breast, Colon or Bladder Carcinoma
Cellular Localization
Cell Surface
Chromosome Location
7p11.2
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis Purified EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R). Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R). Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Placenta stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Placenta stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Placenta stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Placenta stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).)
Related Product Information for anti-EGFR antibody
Recombinant Rabbit Monoclonal Antibody
References
Wikstrand CJ, et al. Cancer Res. 1995 Jul 15; 55(14):3140-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~145kDa (vIII variant)
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
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Product Notes
The EGFR egfr (Catalog #AAA23905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGFRvIII (Epidermal Growth Factor Receptor, Variant III) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's EGFRvIII (Epidermal Growth Factor Receptor, Variant III) can be used in a range of immunoassay formats including, but not limited to, Immunohistology (Formalin-Fixed). Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFRvIII (Epidermal Growth Factor Receptor, Variant III), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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