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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse AMACR/p504S Monoclonal Antibody | anti-AMACR antibody
AMACR/p504S (Prostate Cancer Marker)
Gene Names
AMACR; RM; RACE; CBAS4; P504S; AMACRD
Reactivity
Human. Others not known.
Applications
Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
AMACR/p504S, Antibody; AMACR/p504S (Prostate Cancer Marker); Alpha-methylacyl-CoA Racemase; CBAS4; Da1-8; Macr1; RACE; RM; anti-AMACR antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
AMACR/1864
Specificity
This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
229
Applicable Applications for anti-AMACR antibody
Western Blot (WB), Immunohistology (Formalin-Fixed)
Application Notes
WB: 0.5-1.0ug/ml
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)
Positive Control
HEK Cells or Prostate Adenocarcinoma
Cellular Localization
Cytoplasmic (granular)
Chromosome Location
5p13.3
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864) Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864) Confirmation of Purity and Integrity of Antibody.)
WB (Western Blot)
(Western Blot of Human Prostate Cancer PC3 Cell lysate AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
WB (Western Blot)
(Western Blot of Human Prostate Cancer PC3 Cell lysate AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
WB (Western Blot)
(Western Blot (1) Recombinant AMACR and (2) Human Kidney lysate using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
WB (Western Blot)
(Western Blot (1) Recombinant AMACR and (2) Human Kidney lysate using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)
References
Jiang Z et. al. Human Pathology. 2003; 34(8):792
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42kDa
NCBI Official Full Name
alpha-methylacyl-CoA racemase isoform 3
NCBI Official Synonym Full Names
alpha-methylacyl-CoA racemase
NCBI Official Symbol
AMACR
NCBI Official Synonym Symbols
RM; RACE; CBAS4; P504S; AMACRD
NCBI Protein Information
alpha-methylacyl-CoA racemase
UniProt Protein Name
Alpha-methylacyl-CoA racemase
UniProt Gene Name
AMACR
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Product Notes
The AMACR amacr (Catalog #AAA23906) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AMACR/p504S (Prostate Cancer Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's AMACR/p504S can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistology (Formalin-Fixed). WB: 0.5-1.0ug/ml Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the AMACR amacr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMACR/p504S, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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