Mouse anti-Human Nuclear Factor I/C Monoclonal Antibody | anti-NFATC2 antibody

Nuclear Factor I/C (NFIC, CCAAT-binding Transcription Factor, CTF, Nuclear Factor 1 C-Type, CTF5, MGC20153, NF-I, NF1-C, CTF, TGGCA-Binding Protein, NFI)

Cat. Num. AAA24060

• Gene Names: NFATC2; NFAT1; NFATP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Nuclear Factor I/C; Monoclonal Antibody; Nuclear Factor I/C (NFIC; CCAAT-binding Transcription Factor; CTF; Nuclear Factor 1 C-Type; CTF5; MGC20153; NF-I; NF1-C; TGGCA-Binding Protein; NFI); Anti -Nuclear Factor I/C (NFIC; anti-NFATC2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C12-2A2
• Specificity: Recognizes human NFIC.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSCRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPSWYLG

• Applicable Applications for anti-NFATC2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunofluorescence: 10ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
• Immunogen: Full length recombinant corresponding to aa1-429 from human NFIC (AAH12120) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

WB (Western Blot)

(Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry)

(Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])

WB (Western Blot)

(Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody.Lane 1: NFIC transfected lysate (47.6kD).Lane 2: Non-transfected lysate.)

WB (Western Blot)

(NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)

WB (Western Blot)

(Western Blot detection against Immunogen (72.82kD).)

Related Product Information for anti-NFATC2 antibody

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Product Categories/Family for anti-NFATC2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 27886541
• NCBI GeneID: 4773
• NCBI Accession #: NP_775114.1
• NCBI GenBank Nucleotide #: NM_173091.3
• UniProt Accession #: Q13469; Q13468; Q5TFW7; Q5TFW8; Q9NPX6; Q9NQH3; Q9UJR2; B5B2N8; B5B2N9
• Molecular Weight: 100,146 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 2 isoform C
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• NCBI Official Symbol: NFATC2
• NCBI Official Synonym Symbols: NFAT1; NFATP
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 2; NF-ATc2; NFAT pre-existing subunit; T cell transcription factor NFAT1; T-cell transcription factor NFAT1; NFAT transcription complex, preexisting component; preexisting nuclear factor of activated T-cells 2; nuclear factor of activated T-cells, preexisting component
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 2
• UniProt Gene Name: NFATC2
• UniProt Synonym Gene Names: NFAT1; NFATP; NF-ATc2; NFATc2; NF-ATp
• UniProt Entry Name: NFAC2_HUMAN