Standard Curve (Sample)
Human MMP10 ELISA Kit | MMP10 elisa kit
Human MMP10 ELISA Kit
Gene Names
MMP10; SL-2; STMY2
Reactivity
Human
Synonyms
MMP10; Human MMP10 ELISA Kit; STMY2; Stromelysin-2; SL-2; Matrix metalloproteinase-10; MMP-10; Transin-2; MMP10 elisa kit
Reactivity
Human
Specificity
The Human MMP10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MMP10 proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
476
Samples
Culture Supernates, Serum, Plasma
Sensitivity
< 25 pg/ml
Detection Method
Colorimetric
Preparation and Storage
Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MMP10 elisa kit
Background: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.
Principle of the Assay: The Human MMP10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP10 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human MMP10 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human MMP10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP10 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human MMP10 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,151 Da
NCBI Official Full Name
stromelysin-2 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 10
NCBI Official Symbol
MMP10
NCBI Official Synonym Symbols
SL-2; STMY2
NCBI Protein Information
stromelysin-2
UniProt Protein Name
Stromelysin-2
UniProt Gene Name
MMP10
UniProt Synonym Gene Names
STMY2; SL-2; MMP-10
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Product Notes
The Human MMP10 mmp10 (Catalog #AAA27878) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27878 ELISA Kit recognizes Human MMP10. It is sometimes possible for the material contained within the vial of "MMP10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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