Rabbit anti-Human BCL2 Polyclonal Antibody | anti-BCL2 antibody

BCL2 Antibody

Cat. Num. AAA29670

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BCL2; Polyclonal Antibody; BCL2 Antibody; Bcl-2; ONCOGENEB-CELLLEUKEMIA2; ApoptosisregulatorBcl-2; anti-BCL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BCL2 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-BCL2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human BCL2.
• Target Name: BCL2
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cell using BCL2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded rat lung using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat kidney using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse lung using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver cancer using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Breast cancer using BCL2 Antibody.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using BCL2 antibody.)

Related Product Information for anti-BCL2 antibody

BCL2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within BCL2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of BCL2 may be a marker for mitotic events (4,5). Mutation of BCL2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced BCL2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

Product Categories/Family for anti-BCL2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• UniProt Accession #: P104153