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IF (Immunofluorescence) (Immunofluorescence analysis of PC12 cells using EGFR antibody.)

Rabbit EGFR Polyclonal Antibody | anti-EGFR antibody

EGFR Polyclonal Antibody

Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
EGFR; Polyclonal Antibody; EGFR Polyclonal Antibody; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA; epidermal growth factor receptor; anti-EGFR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 50% glycerol, pH7.4
Applicable Applications for anti-EGFR antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:100
Immunogen
Recombinant fusion protein of human EGFR (NP_005219.2).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of PC12 cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC12 cells using EGFR antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using EGFR antibody.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody.)

WB (Western Blot)

(Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody.)

WB (Western Blot)

(Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody.)

WB (Western Blot) (Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody.)
Related Product Information for anti-EGFR antibody
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Product Categories/Family for anti-EGFR antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,277 Da
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
NCBI Protein Information
epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN

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Product Notes

The EGFR egfr (Catalog #AAA29798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGFR Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:50-1:100. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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