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FCM (Flow Cytometry)
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FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Rabbit UQCRFS1 Monoclonal Antibody | anti-UQCRFS1 antibody

UQCRFS1 Antibody

Gene Names
UQCRFS1; RIP1; RIS1; RISP; UQCR5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
UQCRFS1; Monoclonal Antibody; UQCRFS1 Antibody; Complex III subunit 5 antibody; Complex III subunit IX antibody; Cytochrome b c1 complex subunit Rieske mitochondrial antibody; Cytochrome b-c1 complex subunit 11 antibody; Cytochrome b-c1 complex subunit 5 antibody; Cytochrome b6f complex iron sulfur subunit; chloroplastic antibody; petC antibody; PGR1 antibody; Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody; Proton gradient regulation protein 1 antibody; Rieske iron sulfur protein antibody; Rieske iron-sulfur protein antibody; RIP1 antibody; RIS1 antibody; RISP antibody; Ubiquinol cytochrome C reductase rieske iron sulphur antibody; Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody; Ubiquinol-cytochrome c reductase 8 kDa protein antibody; Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody; UCRI_HUMAN antibody; UQCR5 antibody; UQCRFS1 antibody; anti-UQCRFS1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG62-31
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-UQCRFS1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:500-1:2000
FC/FACS: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human UQCRFS1 aa 100 to the C-terminus.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining UQCRFS1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining UQCRFS1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining UQCRFS1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining UQCRFS1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining UQCRFS1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining UQCRFS1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of UQCRFS1 on different lysates using anti-UQCRFS1 antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: 293 Lane 3: Mouse kidney)

WB (Western Blot) (Western blot analysis of UQCRFS1 on different lysates using anti-UQCRFS1 antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: 293 Lane 3: Mouse kidney)
Related Product Information for anti-UQCRFS1 antibody
Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Incorporation of UQCRFS1 is the penultimate step in complex III assembly.
Product Categories/Family for anti-UQCRFS1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,668 Da
NCBI Official Full Name
cytochrome b-c1 complex subunit Rieske, mitochondrial
NCBI Official Synonym Full Names
ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
NCBI Official Symbol
UQCRFS1
NCBI Official Synonym Symbols
RIP1; RIS1; RISP; UQCR5
NCBI Protein Information
cytochrome b-c1 complex subunit Rieske, mitochondrial; complex III subunit 5; Rieske iron-sulfur protein; cytochrome b-c1 complex subunit 5; ubiquinol-cytochrome c reductase iron-sulfur subunit
UniProt Protein Name
Cytochrome b-c1 complex subunit Rieske, mitochondrial
UniProt Gene Name
UQCRFS1
UniProt Synonym Gene Names
RISP
UniProt Entry Name
UCRI_HUMAN

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Product Notes

The UQCRFS1 uqcrfs1 (Catalog #AAA30490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UQCRFS1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UQCRFS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:500-1:2000 FC/FACS: 1:10-1:50. Researchers should empirically determine the suitability of the UQCRFS1 uqcrfs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UQCRFS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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