Rabbit NFkappaB-p65 Polyclonal Antibody | anti-NFkappaB-p65 antibody

NFkappaB-p65 (Phospho-Ser529) Rabbit Polyclonal Antibody

Cat. Num. AAA30745

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: NFkappaB-p65; Polyclonal Antibody; NFkappaB-p65 (Phospho-Ser529) Rabbit Polyclonal Antibody; RELA; NFkappaB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; BH6842; anti-NFkappaB-p65 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Specificity: Phospho-NFkappaB-p65 (S529) Polyclonal Antibody detects endogenous levels of NFkappaB-p65 protein only when phosphorylated at S529.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NFkappaB-p65 antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
• Application Notes: IF: 1:50-200; WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/20000
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human NFkappaB-p65 (phospho Ser529)
• Modification: Phospho Specific
• Preparation and Storage: Store at -20 degree C/1 year

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

WB (Western Blot)

(Western Blot analysis of various cells using Phospho-NF?B-p65 (S529) Polyclonal Antibody diluted at 1:500)

ELISA

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-?B p65 (Phospho-Ser529) Antibody)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using NF-?B p65 (Phospho-Ser529) Antibody. The picture on the right is blocked with the NF-?B p65 (Phospho-Ser529) peptide.)

Related Product Information for anti-NFkappaB-p65 antibody

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the Cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFkappaB1 or NFkappaB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFkappaB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 60,219 Da
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN