Mouse Caspase-8 Monoclonal Antibody | anti-CASP8 antibody

Caspase-8 Monoclonal Antibody (2G12)

Cat. Num. AAA30819

• Gene Names: CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Caspase-8; Monoclonal Antibody; Caspase-8 Monoclonal Antibody (2G12); Caspase8; CASP8; MCH5; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; anti-CASP8 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 2G12
• Specificity: The antibody detects endogenous Caspase-8 protein.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-CASP8 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-2000; IHC: 1:200-500; IF: 1:200
• Immunogen: Recombinant Protein of Caspase-8
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-liver tissue. 1.Caspase-8 Monoclonal Antibody(2G12)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.ER? Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.Caspase-8 Monoclonal Antibody(2G12) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase-8 Monoclonal Antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Caspase-8 Monoclonal Antibody(2G12) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

WB (Western Blot)

(Western blot analysis of 1) Hela. 2) Mouse Brain Tissue. 3) Rat Brain Tissue using Caspase-8 Monoclonal Antibody.)

Related Product Information for anti-CASP8 antibody

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alt

Product Categories/Family for anti-CASP8 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 122056474
• NCBI GeneID: 841
• NCBI Accession #: NP_001073593.1
• NCBI GenBank Nucleotide #: NM_001080124.1
• UniProt Accession #: Q14790; O14676; Q14791; Q14792; Q14793; Q14794; Q14795; Q14796; Q15780; Q15806; Q53TT5; Q8TDI1
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: caspase-8 isoform C
• NCBI Official Synonym Full Names: caspase 8, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP8
• NCBI Official Synonym Symbols: CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• NCBI Protein Information: caspase-8; FADD-like ICE; MACH-alpha-1/2/3 protein; apoptotic protease Mch-5; MACH-beta-1/2/3/4 protein; apoptotic cysteine protease; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; FADD-homologous ICE/CED-3-like protease; caspase 8, apopto
• UniProt Protein Name: Caspase-8
• UniProt Gene Name: CASP8
• UniProt Synonym Gene Names: MCH5; CASP-8; FLICE; MACH
• UniProt Entry Name: CASP8_HUMAN