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Application Data

Mouse anti-Human MutL Protein Homolog 1 (MLH1) Monoclonal Antibody | anti-MLH1 antibody

MutL Protein Homolog 1 (MLH1)(ABT-MLH1) Antibody

Gene Names
MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Synonyms
MutL Protein Homolog 1 (MLH1); Monoclonal Antibody; MutL Protein Homolog 1 (MLH1)(ABT-MLH1) Antibody; anti-MLH1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
ABT-MLH1
Specificity
This antibody detects endogenous levels of MLH1 protein.
Purity/Purification
The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applicable Applications for anti-MLH1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-100
ELISA: 1:500-5000
Immunogen
Synthesized peptide derived from human MutL Protein Homolog 1 (MLH1) AA range: 400-500
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

Application Data

Application Data

SDS-PAGE

(Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1(ABT-MLH1) antibody. The HRP-conjugated Goat anti-M)

SDS-PAGE (Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1(ABT-MLH1) antibody. The HRP-conjugated Goat anti-M)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)

IHC (Immunohistochemistry) (Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)

IHC (Immunohistochemistry)

(Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)

IHC (Immunohistochemistry) (Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)
Product Categories/Family for anti-MLH1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,601 Da
NCBI Official Full Name
DNA mismatch repair protein Mlh1 isoform 1
NCBI Official Synonym Full Names
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
NCBI Official Symbol
MLH1
NCBI Official Synonym Symbols
FCC2; COCA2; HNPCC; hMLH1; HNPCC2
NCBI Protein Information
DNA mismatch repair protein Mlh1
UniProt Protein Name
DNA mismatch repair protein Mlh1
UniProt Gene Name
MLH1
UniProt Synonym Gene Names
COCA2
UniProt Entry Name
MLH1_HUMAN

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Product Notes

The MLH1 mlh1 (Catalog #AAA30836) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MutL Protein Homolog 1 (MLH1)(ABT-MLH1) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MutL Protein Homolog 1 (MLH1) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-100 ELISA: 1:500-5000. Researchers should empirically determine the suitability of the MLH1 mlh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MutL Protein Homolog 1 (MLH1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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