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WB (Western Blot)
(Western blot analysis of Catenin 1 Antibody expression in MCF-7 cells lysates.The lane on the left is treated with the antigen-specific peptide..The lane on the left is treated with the antigen-specific peptide.)
Rabbit Catenin alpha1 Polyclonal Antibody | anti-CTNNA1 antibody
Catenin alpha1 Antibody
Gene Names
CTNNA1; MDPT2; CAP102
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Catenin alpha1; Polyclonal Antibody; Catenin alpha1 Antibody; Alpha E-catenin; Cadherin associated protein 102kDa; Cadherin associated protein; Cadherin-associated protein; CAP 102; CAP102; Catenin (cadherin associated protein) alpha 1 102kDa; Catenin (cadherin associated protein); alpha 1; 102kDa; Catenin alpha 1; Catenin alpha-1; CTNA1_HUMAN; CTNNA 1; Ctnna1; FLJ36832; FLJ52416; MDPT2; NY REN 13 antigen; OTTHUMP00000224141; OTTHUMP00000224147; Renal carcinoma antigen NY REN 13; Renal carcinoma antigen NY-REN-13; anti-CTNNA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Catenin alpha1 antibody detects endogenous levels of total Catenin alpha1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
841
Applicable Applications for anti-CTNNA1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:200
IHC: 1:50-1:200
IF: 1:200
Immunogen
A synthesized peptide derived from human Catenin alpha1
Subcellular Location
Cell Membrane and Cytoplasm > Cytoskeleton. Cell Junction > Adherens Junction. Cell Membrane. Cell Junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
Tissue Specificity
Expressed ubiquitously in normal tissues.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Catenin 1 Antibody expression in MCF-7 cells lysates.The lane on the left is treated with the antigen-specific peptide..The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Catenin 1 Antibody expression in MCF-7 cells lysates.The lane on the left is treated with the antigen-specific peptide..The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistchemistry)
(Catenin alpha1 Antibody for IHC in human gallbladder tissue.)
IHC (Immunohistchemistry)
(Catenin alpha1 Antibody for IHC in human gallbladder tissue.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using Catenin alpha1 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using Catenin alpha1 Antibody.)
WB (Western Blot)
(Western blot analysis of Catenin alpha1 expression in mouse muscle tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Catenin alpha1 expression in mouse muscle tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Catenin alpha1 expression in various lysates)
WB (Western Blot)
(Western blot analysis of Catenin alpha1 expression in various lysates)
WB (Western Blot)
(Western blot analysis of Catenin alpha1 expression in S3/rat liver tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Catenin alpha1 expression in S3/rat liver tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
Related Product Information for anti-CTNNA1 antibody
Function: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E-and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
Subunit Structure: Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds AFDN and F-actin. Possible component of an E-cadherin/catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma-catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1 (By similarity). Interacts with vinculin/VCL (PubMed:26691986).
Post-translational Modifications: Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.
Similarity: Belongs to the vinculin/alpha-catenin family.
Subunit Structure: Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds AFDN and F-actin. Possible component of an E-cadherin/catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma-catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1 (By similarity). Interacts with vinculin/VCL (PubMed:26691986).
Post-translational Modifications: Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.
Similarity: Belongs to the vinculin/alpha-catenin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 100 kDa
Predicted: 101 kDa
Predicted: 101 kDa
NCBI Official Full Name
catenin alpha-1 isoform 2
NCBI Official Synonym Full Names
catenin alpha 1
NCBI Official Symbol
CTNNA1
NCBI Official Synonym Symbols
MDPT2; CAP102
NCBI Protein Information
catenin alpha-1
UniProt Protein Name
Catenin alpha-1
UniProt Gene Name
CTNNA1
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Product Notes
The CTNNA1 ctnna1 (Catalog #AAA31080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Catenin alpha1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Catenin alpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:200. Researchers should empirically determine the suitability of the CTNNA1 ctnna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catenin alpha1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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