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CD3, Monoclonal Antibody (Cat# AAA12050)

Full Name

RAT ANTI MOUSE CD3:RPE

Gene Names

Cd3e; CD3; T3e; AI504783; CD3epsilon

Applications

Flow cytometry (FC/FACS)

Pricing

Prostaglandin E1 (PGE1), ELISA Kit (Cat# AAA13074)

Full Name

Rat Prostaglandin E1 (PGE1) ELISA Kit

Gene Names

SLCO3A1; OATPD; OATP-D; OATP3A1; SLC21A11

Reactivity

Rat

Pricing

parathyroid hormone (PTH), ELISA Kit (Cat# AAA15634)

Full Name

Mouse parathyroid hormone, PTH ELISA Kit

Gene Names

PTH; PTH1

Reactivity

Mouse

Pricing

circulating immune complex (CIC), ELISA Kit (Cat# AAA15890)

Full Name

Dog circulating immune complex, CIC ELISA Kit

Reactivity

Dog

Pricing

Total bile acide, ELISA Kit (Cat# AAA16146)

Full Name

Human Total bile acide ELISA Kit

Reactivity

Human

Pricing

Nicotinic Acetylcholine Receptor, ELISA Kit (Cat# AAA17426)

Full Name

Rat Nicotinic Acetylcholine Receptor ELISA Kit

Gene Names

CHRNA4; EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4

Reactivity

Rat

Pricing

Tartrate Resistant Acid Phosphatase (TRAP), ELISA Kit (Cat# AAA18962)

Full Name

Mouse Tartrate Resistant Acid Phosphatase (TRAP) ELISA Kit

Reactivity

Mouse

Pricing

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Application Data (Detection limit for recombinant GST tagged SPP1 is ~1ng/ml as a capture antibody.)

Osteopontin, Monoclonal Antibody (Cat# AAA24594)

Full Name

Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) APC

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in PC-12.)

LIMK1, Monoclonal Antibody (Cat# AAA24850)

Full Name

LIMK1 (LIM Domain Kinase 1, LIMK-1, LIMK) (Biotin)

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)

G3BP1, Monoclonal Antibody (Cat# AAA25106)

Full Name

G3BP1 (Ras GTPase-activating Protein-binding Protein 1, G3BP-1, ATP-dependent DNA Helicase VIII, hDH VIII, GAP SH3 Domain-binding Protein 1, G3BP) (FITC)

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RNF2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of RNF2 expression in Raw 264.7.)

RNF2, Monoclonal Antibody (Cat# AAA26130)

Full Name

RNF2 (RING Finger Protein 2, BAP-1, BAP1, DING, HIPI3, Ring1B, Ring2) (APC)

Gene Names

RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged DDX56 is 0.1 ng/ml as a capture antibody.)

DDX56, Monoclonal Antibody (Cat# AAA26386)

Full Name

DDX56 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56, DDX21, DDX26, NOH61) (FITC)

Gene Names

DDX56; DDX21; DDX26; NOH61

Applications

Western Blot (WB)

Purity

Purified

Pricing

Lipofuscin, ELISA Kit (Cat# AAA27154)

Full Name

Mouse Lipofuscin ELISA Kit

Reactivity

Mouse

Pricing

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EIF2C3/Argonaute 3, Monoclonal Antibody (Cat# AAA30227)

Full Name

EIF2C3/Argonaute 3 Antibody

Gene Names

AGO3; EIF2C3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

Dopamine (DA), ELISA Kit (Cat# AAA31507)

Full Name

Human Dopamine (DA) ELISA Kit

Reactivity

Human

Pricing

Semaphorin 3C (SEMA3C), ELISA Kit (Cat# AAA11284)

Full Name

Human Semaphorin 3C (SEMA3C) ELISA Kit

Gene Names

SEMA3C; SemE; SEMAE

Reactivity

Human

Pricing

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Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)

CD43, Monoclonal Antibody (Cat# AAA12052)

Full Name

MOUSE ANTI RAT CD43:RPE

Gene Names

Spn; Lsn; Cd43; Lsn1; gpL115

Applications

Flow cytometry (FC/FACS)

Pricing

Fecal Calprotectin, ELISA Kit (Cat# AAA14612)

Full Name

Fecal Calprotectin, Human, Rapid ELISA kit

Reactivity

Human

Pricing

interferon, beta 1, fibroblast, ELISA Kit (Cat# AAA15124)

Full Name

Mouse Interferon beta, IFN-beta/IFNB ELISA Kit

Gene Names

Ifnb1; Ifb; IFNB; IFN-beta

Reactivity

Mouse

Pricing

complement fragment 3b (C3b), ELISA Kit (Cat# AAA15892)

Full Name

Mouse complement fragment 3b, C3b ELISA Kit

Reactivity

Mouse

Pricing

hypoxia-inducible factor 1alpha, ELISA Kit (Cat# AAA16404)

Full Name

Rat hypoxia-inducible factor 1alpha ELISA Kit

Gene Names

HIF1A; HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA

Reactivity

Rat

Pricing

Vitamin D Receptor, ELISA Kit (Cat# AAA16660)

Full Name

Mouse Vitamin D Receptor ELISA Kit

Reactivity

Mouse

Pricing

Platelet activating factor, ELISA Kit (Cat# AAA16916)

Full Name

Monkey Platelet activating factor ELISA Kit

Reactivity

Monkey

Pricing

Epinephrine/Adrenaline, ELISA Kit (Cat# AAA17428)

Full Name

Epinephrine/Adrenaline ELISA Kit

Reactivity

General

Pricing

delta-like 1 homolog (Drosophila), ELISA Kit (Cat# AAA18196)

Full Name

Mouse Protein delta homolog 1, DLK1 ELISA Kit

Gene Names

Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678

Reactivity

Mouse

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-PRPF18 antibody (AAA19988).Overlay histogram showing THP-1 cells stained with AAA19988 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF18 Antibody (AAA19988, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PRPF18 using anti-PRPF18 antibody (AAA19988).PRPF18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF18 antigen affinity purified polyclonal antibody (#AAA19988) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (42 kDa.)

PRPF18, Polyclonal Antibody (Cat# AAA19988)

Full Name

Anti-PRPF18 Antibody Picoband

Gene Names

PRPF18; PRP18; hPrp18

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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RAR Related Orphan Receptor Gamma (RORg), Recombinant Protein (Cat# AAA20244)

Full Name

Recombinant RAR Related Orphan Receptor Gamma (RORg)

Gene Names

RORC; TOR; RORG; RZRG; NR1F3; RZR-GAMMA

Applications

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Purity

>95%

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Lamin A/C, Polyclonal Antibody (Cat# AAA22292)

Full Name

Lamin A/C Polyclonal Antibody

Gene Names

LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in PC-12.)

KLF10, Monoclonal Antibody (Cat# AAA26132)

Full Name

KLF10 (Kruppel-like Factor 10, EGRA, TIEG, TIEG1) (APC)

Gene Names

KLF10; EGRA; TIEG; TIEG1; EGR-alpha

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified

Pricing

soluble OX40 Ligand, ELISA Kit (Cat# AAA27156)

Full Name

Monkey soluble OX40 Ligand ELISA Kit

Reactivity

Monkey

Pricing

Soluble Interleukin 2a receptor, ELISA Kit (Cat# AAA16410)

Full Name

Mouse Soluble Interleukin 2a receptor ELISA Kit

Gene Names

IL2RA; p55; CD25; IL2R; TCGFR; IDDM10

Reactivity

Mouse

Pricing

Leukocyte antigen E, ELISA Kit (Cat# AAA17178)

Full Name

Mouse Leukocyte antigen E ELISA Kit

Gene Names

HLA-E; MHC; QA1; EA1.2; EA2.1; HLA-6.2

Reactivity

Mouse

Pricing

beta 2 transferrin (beta2TF), ELISA Kit (Cat# AAA18202)

Full Name

Human beta 2 transferrin, beta2TF ELISA Kit

Reactivity

Human

Pricing

Wilms tumor protein homolog (Wt1), Recombinant Protein (Cat# AAA18458)

Full Name

Recombinant Mouse Wilms tumor protein homolog (Wt1)

Gene Names

Wt1; Wt-1; D630046I19Rik

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-SOS1 antibody (AAA19738).Overlay histogram showing U87 cells stained with AAA19738 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SOS1 Antibody (AAA19738, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of SOS1 using anti-SOS1 antibody (AAA19738).SOS1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SOS1 Antibody (AAA19738) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SOS1 Antibody (AAA19738) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SOS1 Antibody (AAA19738) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SOS1 Antibody (AAA19738) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SOS1 Antibody (AAA19738) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SOS1 Antibody (AAA19738) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human THP-1 whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOS1 antigen affinity purified polyclonal antibody (#AAA19738) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOS1 at approximately 170 kDa. The expected band size for SOS1 is at 152 kDa.)

SOS1, Polyclonal Antibody (Cat# AAA19738)

Full Name

Anti-SOS1 Antibody Picoband

Gene Names

SOS1; GF1; HGF; NS4; GGF1; GINGF

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse pancreas cells using Pro-glucagon Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Pro-glucagon, Polyclonal Antibody (Cat# AAA22298)

Full Name

Pro-glucagon Polyclonal Antibody

Gene Names

GCG; GLP1; GLP2; GRPP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Application Data (Detection limit for recombinant GST tagged PITPNA is 0.1ng/ml as a capture antibody.)

PITPNA, Monoclonal Antibody (Cat# AAA24090)

Full Name

PITPNA (Phosphatidylinositol Transfer Protein alpha Isoform, PI-TP-alpha, PtdIns Transfer Protein alpha, PtdInsTP alpha, PITPN, MGC99649)

Gene Names

PITPNA; PITPN; VIB1A; PI-TPalpha

Reactivity

Human, Mouse, Rat

Applications

ELISA (EL/EIA), Western Blot (WB)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in A-431.)

RRAS2, Monoclonal Antibody (Cat# AAA24346)

Full Name

RRAS2 (Ras-related Protein R-Ras2, Ras-like Protein TC21, Teratocarcinoma Oncogene, TC21) (AP)

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

MCM2, Monoclonal Antibody (Cat# AAA24858)

Full Name

MCM2 (DNA Replication Licensing Factor MCM2, Minichromosome Maintenance Protein 2 Homolog, Nuclear Protein BM28, BM28, CCNL1, CDCL1, KIAA0030, MGC10606) (Biotin)

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (GNRHR2 monoclonal antibody, Western Blot analysis of GNRHR2 expression in HeLa.)

GNRHR2, Monoclonal Antibody (Cat# AAA25114)

Full Name

GNRHR2 (Putative Gonadotropin-releasing Hormone II Receptor, GnRH II Receptor, GnRH-II-R, Type II GnRH Receptor) (FITC)

Gene Names

FZD2; Fz2; fz-2; fzE2; hFz2; OMOD2

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.)

BST2, Monoclonal Antibody (Cat# AAA26138)

Full Name

BST2 (Bone Marrow Stromal Cell antigen 2, CD317) (APC)

Gene Names

BST2; CD317; TETHERIN

Applications

Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified

Pricing

Thioredoxin-Interacting Protein (TXNIP), ELISA Kit (Cat# AAA31514)

Full Name

Mouse Thioredoxin-Interacting Protein (TXNIP) ELISA Kit

Gene Names

Txnip; THIF; Tbp-2; VDUP1; Hyplip1; AA682105; 1200008J08Rik

Reactivity

Mouse

Pricing

kappa immunoglobulin light chain (kappa-IgLC), ELISA Kit (Cat# AAA12827)

Full Name

Human kappa immunoglobulin light chain (kappa-IgLC) ELISA Kit

Reactivity

Human

Pricing

apolipoprotein E, ELISA Kit (Cat# AAA15131)

Full Name

Rat apolipoprotein E, Apo-E ELISA Kit

Reactivity

Rat

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-RANBP2 antibody (AAA19742).Overlay histogram showing MCF-7 cells stained with AAA19742 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RANBP2 Antibody (AAA19742, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RANBP2 using anti-RANBP2 antibody (AAA19742).RANBP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP2 antigen affinity purified polyclonal antibody (#AAA19742) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANBP2 at approximately 358 kDa. The expected band size for RANBP2 is at 358 kDa.)

RANBP2, Polyclonal Antibody (Cat# AAA19742)

Full Name

Anti-RANBP2 Antibody Picoband

Gene Names

RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEL cells using anti-NUDT19 antibody (AAA19998).Overlay histogram showing HEL cells stained with AAA19998 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUDT19 Antibody (AAA19998, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of NUDT19 using anti-NUDT19 antibody (AAA19998).NUDT19 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUDT19 Antibody (AAA19998) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUDT19 Antibody (AAA19998) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUDT19 Antibody (AAA19998) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUDT19 Antibody (AAA19998) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT19 antigen affinity purified polyclonal antibody (#AAA19998) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUDT19 at approximately 40 kDa. The expected band size for NUDT19 is at 40 kDa.)

NUDT19, Polyclonal Antibody (Cat# AAA19998)

Full Name

Anti-NUDT19 Antibody Picoband

Gene Names

NUDT19; RP2

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines.)

PSMA5, Polyclonal Antibody (Cat# AAA21278)

Full Name

Anti-PSMA5 Antibody

Gene Names

PSMA5; PSC5; ZETA

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Affinity Purified

Pricing

Cross Linked C-Telopeptide Of Type II Collagen (CTXII), ELISA Kit (Cat# AAA22814)

Full Name

Human Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ELISA Kit

Reactivity

Human

Pricing

S100A4, Monoclonal Antibody (Cat# AAA24350)

Full Name

S100A4 (Protein S100-A4, Calvasculin, Metastasin, Placental Calcium-binding Protein, Protein Mts1, S100 Calcium-binding Protein A4, CAPL, MTS1) (AP)

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

GSR, Monoclonal Antibody (Cat# AAA25118)

Full Name

GSR (Glutathione Reductase, Mitochondrial, GR, GRase, GSR, GLUR, GRD1, MGC78522) (FITC)

Gene Names

GSR; HEL-75; HEL-S-122m

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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