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WB (Western Blot) (Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD)

MARCH7, Polyclonal Antibody (Cat# AAA28826)

Full Name

MARCH7 Polyclonal Antibody

Gene Names

MARCH7; AXO; AXOT; RNF177; MARCH-VII

Reactivity

Rat, Pig, Dog, Cow

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Lamin B1, Polyclonal Antibody (Cat# AAA29060)

Full Name

Lamin B1 Polyclonal Antibody

Gene Names

LMNB1; LMN; ADLD; LMN2; LMNB

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

FoxO1, Polyclonal Antibody (Cat# AAA29026)

Full Name

FoxO1 Polyclonal Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

GEF H1, Monoclonal Antibody (Cat# AAA30493)

Full Name

GEF H1 Antibody

Gene Names

ARHGEF2; GEF; P40; GEFH1; LFP40; GEF-H1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Choline Acetyltransferase, Monoclonal Antibody (Cat# AAA30341)

Full Name

Choline Acetyltransferase Antibody

Gene Names

CHAT; CMS1A; CMS1A2; CHOACTASE

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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RSK1/2/3/4, Polyclonal Antibody (Cat# AAA31315)

Full Name

Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Niban, Polyclonal Antibody (Cat# AAA31335)

Full Name

Niban Antibody

Gene Names

FAM129A; GIG39; NIBAN; C1orf24

Reactivity

Human, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

CREB, Polyclonal Antibody (Cat# AAA31303)

Full Name

Phospho-CREB (Ser129) Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

S100A9, Polyclonal Antibody (Cat# AAA31466)

Full Name

Phospho-S100A9 (Thr113) Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300)

JNK1/2/3, Polyclonal Antibody (Cat# AAA28934)

Full Name

JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)

Stat3, Polyclonal Antibody (Cat# AAA28941)

Full Name

Stat3 (phospho Ser727) Polyclonal Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

GTBP, Polyclonal Antibody (Cat# AAA29034)

Full Name

GTBP Polyclonal Antibody

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human, Mouse, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200)

pS2, Polyclonal Antibody (Cat# AAA30757)

Full Name

pS2 Rabbit Polyclonal Antibody

Gene Names

TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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Application Data (Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)

EMR1, Monoclonal Antibody (Cat# AAA26723)

Full Name

EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (AP)

Reactivity

Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Rab 5A, Polyclonal Antibody (Cat# AAA29261)

Full Name

Rab 5A Polyclonal Antibody

Gene Names

RAB5A; RAB5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

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Application Data

Claudin 4, Monoclonal Antibody (Cat# AAA30892)

Full Name

Claudin 4 (ABT-CLD4) Antibody

Gene Names

CLDN4; CPER; CPE-R; CPETR; CPETR1; WBSCR8; hCPE-R

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

Free Fatty Acid (FFA), ELISA Kit (Cat# AAA12946)

Full Name

Porcine Free Fatty Acid (FFA) ELISA Kit

Gene Names

FFAR2; FFA2R; GPR43

Reactivity

Porcine

Pricing

S100 calcium binding protein A4, ELISA Kit (Cat# AAA18131)

Full Name

Human Protein S100-A4, S100A4 ELISA Kit

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human

Pricing

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Application Data (Staining of mouse thymus with Rat anti Mouse CD150: RPE)

CD150, Monoclonal Antibody (Cat# AAA12038)

Full Name

RAT ANTI MOUSE CD150:RPE

Gene Names

Slamf1; Slam; CD150; IPO-3; CDw150; ESTM51; AA177906; 4933415F16

Applications

Flow Cytometry

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of RH35 cells using anti-Tropomyosin 2/TPM2 antibody (AAA19476).Overlay histogram showing RH35 cells stained with AAA19476 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (AAA19476, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Tropomyosin 2/TPM2, Polyclonal Antibody (Cat# AAA19476)

Full Name

Anti-Tropomyosin 2/TPM2 Antibody Picoband

Gene Names

TPM2; DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FOX2/RBM9, Monoclonal Recombinant Antibody (Cat# AAA23868)

Full Name

Rabbit anti-FOX2/RBM9 Recombinant Monoclonal Antibody [BLR215K]

Gene Names

RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 600ug extracts of Mouse brain usingug N-Cadherin Polyclonal Antibody. Western blot was performed from the immunoprecipitate using N-Cadherin Polyclonal Antibody at a dilution of 1:1000.)

N-Cadherin, Polyclonal Antibody (Cat# AAA22314)

Full Name

(KO Validated) N-Cadherin Polyclonal Antibody

Gene Names

CDH2; CDHN; NCAD; CD325; CDw325

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

NDN, Monoclonal Antibody (Cat# AAA24579)

Full Name

NDN (Necdin) APC

Gene Names

NDN; PWCR; HsT16328

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (AKAP8 monoclonal antibody (Western Blot analysis of AKAP8 expression in HeLa NE)

AKAP8, Monoclonal Antibody (Cat# AAA24426)

Full Name

AKAP8 (A-kinase Anchor Protein 8, AKAP-8, A-kinase Anchor Protein 95kD, AKAP 95, AKAP95, DKFZp586B1222) APC

Gene Names

AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody. Lane 1: NQO1 transfected lysate (30.9kD). Lane 2: Non-transfected lysate.)

NQO1, Monoclonal Antibody (Cat# AAA25181)

Full Name

NQO1 (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, QR1, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, DIA4, NMOR1) (FITC)

Gene Names

NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged SPP1 is ~1ng/ml as a capture antibody.)

Osteopontin, Monoclonal Antibody (Cat# AAA25480)

Full Name

Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) (HRP)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Proximity Ligation Analysis of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Phospholipase C, gamma 1, Monoclonal Antibody (Cat# AAA25497)

Full Name

Phospholipase C, gamma 1 (Phospholipase C-gamma-1, PLCgamma1, PLC-gamma-1, PLCg1, NCKAP3, 1-Phosphatidylinositol-4,5-bisphosphate Phosphodiesterase gamma-1, Phospholipase C-II, PLC-II, Phosphoinositide Phospholipase C-gamma-1, PLC1, PLC148, PLC-148) (HRP)

Gene Names

PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (CA1 monoclonal antibody, Western Blot analysis of CA1 expression in HeLa.)

CA1, Monoclonal Antibody (Cat# AAA25628)

Full Name

CA1 (Carbonic Anhydrase 1, Carbonic Anhydrase I, CA-I, Carbonate Dehydratase I, Carbonic Anhydrase B, CAB) (PE)

Gene Names

CA1; CAB; CA-I; Car1; HEL-S-11

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)

HSPA1A, Monoclonal Antibody (Cat# AAA25719)

Full Name

HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (PE)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])

TAF11, Monoclonal Antibody (Cat# AAA26485)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (HRP)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

JAK1, Polyclonal Antibody (Cat# AAA28931)

Full Name

JAK1 (phospho Tyr1022) Polyclonal Antibody

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

JNK1/2/3, Polyclonal Antibody (Cat# AAA28955)

Full Name

JNK1/2/3 (phospho Tyr185) Polyclonal Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

HSPBAP1, Polyclonal Antibody (Cat# AAA29153)

Full Name

HSPBAP1 Polyclonal Antibody

Gene Names

HSPBAP1; PASS1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA29020)

Full Name

ERK 1/2 Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using AKR1C2 Rabbit pAb.)

AKR1C2, Polyclonal Antibody (Cat# AAA30589)

Full Name

AKR1C2 Rabbit Polyclonal Antibody

Gene Names

AKR1C2; DD; DD2; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000)

Perforin 1, Polyclonal Antibody (Cat# AAA29334)

Full Name

Perforin 1 Polyclonal Antibody

Gene Names

PRF1; P1; PFP; FLH2; PFN1; HPLH2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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TDP43, Polyclonal Antibody (Cat# AAA31323)

Full Name

Phospho-TDP43 (Ser409/Ser410) Antibody

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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HP1 alpha, Polyclonal Antibody (Cat# AAA31332)

Full Name

Phospho-HP1 alpha (Ser92) Antibody

Gene Names

CBX5; HP1; HP1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western blot analysis of Phospho-Zyxin (Tyr316) in lysates of Jurkat paclitaxel 1uM 24h, using Phospho-Zyxin (Tyr316) Antibody.)

Zyxin, Polyclonal Antibody (Cat# AAA31158)

Full Name

Phospho-Zyxin (Tyr316) Antibody

Gene Names

ZYX; ESP-2; HED-2

Reactivity

Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Pricing

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CD11b, Monoclonal Antibody (Cat# AAA11971)

Full Name

MOUSE ANTI RAT CD11b

Gene Names

ITGAM; CD11B

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.)

ACLY, Monoclonal Antibody (Cat# AAA14127)

Full Name

Anti-ACLY Mouse mAb

Gene Names

ACLY; ACL; ATPCL; CLATP

Reactivity

Human, Mouse, Monkey, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-GBP1 antibody (AAA19787).Overlay histogram showing MCF-7 cells stained with AAA19787 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GBP1 Antibody (AAA19787, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IP (Immunoprecipitation) (Figure 6. Immunoprecipitating GBP1 in Hela whole cell lysate .Western blot analysis of GBP1 using anti-GBP1 antibody (AAA19787).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-GBP1 antibody in Hela whole cell lysate.Lane 3: anti-GBP1 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GBP1 antigen affinity purified polyclonal antibody (AAA19787) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GBP1 Antibody (AAA19787) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GBP1 Antibody (AAA19787) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GBP1 Antibody (AAA19787) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HUVEC whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat spleen tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse spleen tissue lysayes.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBP1 antigen affinity purified polyclonal antibody (#AAA19787) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GBP1 at approximately 68 kDa. The expected band size for GBP1 is at 68 kDa.)

GBP1, Polyclonal Antibody (Cat# AAA19787)

Full Name

Anti-GBP1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Anti-CRFR1 / CRHR1 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

CRFR1 / CRHR1, Polyclonal Antibody (Cat# AAA12338)

Full Name

Rabbit Polyclonal to Human CRFR1 / CRHR1

Gene Names

CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1

Reactivity

Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat
Predicted Reactivity: Bat, Bovine, Horse (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

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WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)

ALB, Monoclonal Antibody (Cat# AAA24136)

Full Name

ALB (Albumin, Serum Albumin, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341) (AP)

Gene Names

ALB; HSA; PRO0883; PRO0903; PRO1341

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

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WB (Western Blot) (Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)