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regenerating islet-derived 3 gamma, ELISA Kit (Cat# AAA18070)

Full Name

Human Regenerating islet-derived protein 3-gamma, REG3G ELISA Kit

Reactivity

Human

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ELISA (Detection limit for recombinant GST tagged SERPING1 is approximately 0.1 ng/ml as a capture antibody.)

SERPING1 / C1 Inhibitor, Monoclonal Antibody (Cat# AAA12353)

Full Name

Mouse Monoclonal [clone 3F4-1D9] (IgG2a,k) to Human SERPING1 / C1 Inhibitor

Gene Names

SERPING1; C1IN; C1NH; HAE1; HAE2; C1INH

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Purity

Protein A Purified

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Transfection Control (Anti-GPR182 / ADMR antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

GPR182 / ADMR, Polyclonal Antibody (Cat# AAA12333)

Full Name

Rabbit Polyclonal to Human GPR182 / ADMR

Gene Names

GPR182; AMR; 7TMR; ADMR; AM-R; G10D; gamrh; hrhAMR

Reactivity

Gorilla, Human, Monkey
Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry, Immunocytochemistry

Purity

Immunoaffinity Purified

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using p70 S6 Kinase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

p70 S6 Kinase 1, Polyclonal Antibody (Cat# AAA22303)

Full Name

p70 S6 Kinase 1 Polyclonal Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)

Progesterone Receptor, Monoclonal Antibody (Cat# AAA24918)

Full Name

Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3) (Biotin)

Gene Names

PGR; PR; NR3C3

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

MUTYH, Monoclonal Antibody (Cat# AAA25463)

Full Name

MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (HRP)

Gene Names

MUTYH; MYH

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data

CD2, Monoclonal Antibody (Cat# AAA26805)

Full Name

CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (MaxLight 550)

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ATP2B1, Monoclonal Antibody (Cat# AAA26691)

Full Name

ATP2B1 (Plasma Membrane Calcium-transporting ATPase 1, PMCA1, Plasma Membrane Calcium ATPase Isoform 1, Plasma Membrane Calcium Pump Isoform 1, PMCA1) (MaxLight 550)

Gene Names

ATP2B1; PMCA1; PMCA1kb

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

Dapper Homolog 1 (DACT1), ELISA Kit (Cat# AAA31500)

Full Name

Human Dapper Homolog 1 (DACT1) ELISA Kit

Gene Names

DACT1; DPR1; TBS2; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1

Reactivity

Human

Pricing

Hepcidin, ELISA Kit (Cat# AAA16721)

Full Name

Bovine Hepcidin ELISA Kit

Gene Names

HAMP; HEPC; PLTR; HFE2B; LEAP1

Reactivity

Bovine

Pricing

Corticotropin Releasing Hormone (CRH), ELISA Kit (Cat# AAA16575)

Full Name

Human Corticotropin Releasing Hormone (CRH) ELISA Kit

Reactivity

Human

Pricing

Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A), ELISA Kit (Cat# AAA15576)

Full Name

Human Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A) ELISA Kit

Gene Names

REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1; MGC12447

Reactivity

Human

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Filamin B/FLNB, Polyclonal Antibody (Cat# AAA19463)

Full Name

Anti-Filamin B/FLNB Antibody Picoband

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.)

ZEB1, Monoclonal Antibody (Cat# AAA14140)

Full Name

Anti-ZEB1 Mouse mAb

Gene Names

ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Pricing

LINE-1, Polyclonal Antibody (Cat# AAA14722)

Full Name

LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)

Gene Names

L1TD1; ECAT11; RP5-1155K23.3

Reactivity

Human

Applications

Western Blot

Purity

Purified
Purified by selective precipitation and salt fractionation followed by extensive dialysis.

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of 293T cells using anti-PTCD1 antibody (AAA19983).Overlay histogram showing 293T cells stained with AAA19983 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTCD1 Antibody (AAA19983, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of PTCD1 using anti-PTCD1 antibody (AAA19983).PTCD1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SIHA whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat heart tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCD1 antigen affinity purified polyclonal antibody (#AAA19983) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTCD1 at approximately 79 kDa. The expected band size for PTCD1 is at 79 kDa.)

PTCD1, Polyclonal Antibody (Cat# AAA19983)

Full Name

Anti-PTCD1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human COL4a4 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))

Collagen Type IV Alpha 4, Polyclonal Antibody (Cat# AAA21020)

Full Name

Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody

Gene Names

COL4A4; CA44

Reactivity

Human

Applications

Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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WB (Western Blot) (RAD18 monoclonal antibody, Western Blot analysis of RAD18 expression in Hela NE.)

RAD18, Monoclonal Antibody (Cat# AAA25222)

Full Name

RAD18 (E3 Ubiquitin-protein Ligase RAD18, Postreplication Repair Protein RAD18, hRAD18, hHR18, RING Finger Protein 73, RNF73) (FITC)

Gene Names

RAD18; RNF73

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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MSH6, Monoclonal Antibody (Cat# AAA27721)

Full Name

Recombinant Anti-MSH6 Antibody, Rabbit Monoclonal

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human, Cynomolgus, Mouse, Rat, Ferret

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Protein A

Pricing

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WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)

ACTB, Monoclonal Antibody (Cat# AAA26656)

Full Name

ACTB (Actin, beta, PS1TP5BP1) (PE)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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ICC (Immunocytochemistry) (ICC staining HSP70 in SKBR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

HSP70, Polyclonal Antibody (Cat# AAA29967)

Full Name

HSP70 Antibody

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Peptide affinity purified

Pricing

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Prohibitin, Monoclonal Antibody (Cat# AAA29994)

Full Name

Prohibitin Antibody

Gene Names

PHB; PHB1

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Smad5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

SMAD5, Monoclonal Antibody (Cat# AAA30038)

Full Name

SMAD5 Antibody

Gene Names

SMAD5; DWFC; JV5-1; MADH5

Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

p57, Polyclonal Antibody (Cat# AAA29226)

Full Name

p57 Polyclonal Antibody

Gene Names

CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Inhibin beta-A, Polyclonal Antibody (Cat# AAA29247)

Full Name

Inhibin beta-A Polyclonal Antibody

Gene Names

INHBA; EDF; FRP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

MYH6/MYH7, Polyclonal Antibody (Cat# AAA29387)

Full Name

MYH6/MYH7 Polyclonal Antibody

Gene Names

MYH6; ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

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FCM (Flow Cytometry) (Overlay Peak curve showing 293F cells transfected with GST stained with AAA28065 (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

GST, Monoclonal Antibody (Cat# AAA28065)

Full Name

GST Monoclonal Antibody

Reactivity

All

Applications

Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation

Purity

>95%, Protein A purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ABCB7 antibody at dilution of 1:100 (40x lens).)

ABCB7, Polyclonal Antibody (Cat# AAA28279)

Full Name

ABCB7 Polyclonal Antibody

Gene Names

ABCB7; ABC7; ASAT; Atm1p; EST140535

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

Tannin, Assay Kit (Cat# AAA27823)

Full Name

Tannin Microplate Assay Kit

Reactivity

General

Applications

Functional Assay

Pricing

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ICC (Immunocytochemistry) (ICC staining GST3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

GST3, Monoclonal Antibody (Cat# AAA30466)

Full Name

GST3 Antibody

Gene Names

GSTP1; PI; DFN7; GST3; GSTP; FAEES3

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

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hUPF1, Monoclonal Antibody (Cat# AAA30506)

Full Name

hUPF1 Antibody

Gene Names

UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-colon tissue. 1.CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

CD4, Monoclonal Antibody (Cat# AAA30811)

Full Name

CD4 Monoclonal Antibody (11A1)

Gene Names

CD4; CD4mut

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody at dilution of 1:100 .)

KRT14, Polyclonal Antibody (Cat# AAA30574)

Full Name

KRT14 Polyclonal Antibody

Gene Names

KRT14; K14; NFJ; CK14; EBS3; EBS4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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ICC (Immunocytochemistry) (ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Actin, Monoclonal Antibody (Cat# AAA30249)

Full Name

Actin Antibody

Gene Names

ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

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Usp14, Monoclonal Antibody (Cat# AAA30408)

Full Name

Usp14 Antibody

Gene Names

USP14; TGT

Reactivity

Human, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

AR, Polyclonal Antibody (Cat# AAA31420)

Full Name

Phospho-AR (Tyr535) Antibody

Gene Names

AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various celllines, using GSK3 alpha/beta Antibody.)

GSK3 alpha/beta, Polyclonal Antibody (Cat# AAA31094)

Full Name

GSK3 alpha/beta Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IRS-1, Polyclonal Antibody (Cat# AAA28930)

Full Name

IRS-1 (phospho Ser636) Polyclonal Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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FCM (Flow Cytometry) (HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

HSPA5, Polyclonal Antibody (Cat# AAA28738)

Full Name

HSPA5 Antibody

Gene Names

HSPA5; BIP; MIF2; GRP78; HEL-S-89n

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Ku-70, Polyclonal Antibody (Cat# AAA28848)

Full Name

Ku-70 (Acetyl Lys542) Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Transferrin Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Transferrin (CD71), Monoclonal Antibody (Cat# AAA30263)

Full Name

Transferrin Receptor (CD71) Antibody

Gene Names

TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

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Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:RPE)

CD28, Monoclonal Antibody (Cat# AAA26768)

Full Name

CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (PE)

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Purity

Purified by Protein G Affinity Chromatography

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

CD85g, Polyclonal Antibody (Cat# AAA29200)

Full Name

CD85g Polyclonal Antibody

Gene Names

LILRA4; ILT7; CD85g

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

OTUB1, Polyclonal Antibody (Cat# AAA31284)

Full Name

Phospho-OTUB1 (Ser16) Antibody

Gene Names

OTUB1; OTB1; OTU1; HSPC263

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

Myoglobin, ELISA Kit (Cat# AAA13363)

Full Name

Human Myoglobin ELISA Kit

Gene Names

MB; PVALB; myoglobgin

Reactivity

Human

Pricing

Entamoeba histolytica Antigen, ELISA Kit (Cat# AAA22678)

Full Name

Human Entamoeba histolytica Antigen (EH Ag) ELISA Kit

Reactivity

Human

Pricing

Allograft Inflammatory Factor 1 (AIF1), Antibody Pair Kit (Cat# AAA21090)

Full Name

Human Allograft Inflammatory Factor 1 (AIF1) Antibody Pair Kit (with Standard)

Gene Names

Aif1; iba1; Bart1; mrf-1; BART-1

Reactivity

Homo sapiens (Human)

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific Affinity Chromatography.

Pricing

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Application Data

Ep-CAM /CD326, Monoclonal Antibody (Cat# AAA13831)

Full Name

Ep-CAM /CD326 (Epithelial Marker) Mouse Monoclonal Antibody

Gene Names

EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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TFEB, Antibody (Cat# AAA17972)

Full Name

TFEB Antibody

Gene Names

TFEB; TCFEB; BHLHE35; ALPHATFEB

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U87 cells using anti-Nesprin3/SYNE3 antibody (AAA19982).Overlay histogram showing U87 cells stained with AAA19982 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin3/SYNE3 Antibody (AAA19982, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (AAA19982).Nesprin3/SYNE3 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin3/SYNE3 Antibody (AAA19982) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin3/SYNE3 Antibody (AAA19982) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin3/SYNE3 Antibody (AAA19982) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Nesprin3/SYNE3 Antibody (AAA19982) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin3/SYNE3 antigen affinity purified polyclonal antibody (#AAA19982) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nesprin3/SYNE3 at approximately 100 kDa. The expected band size for Nesprin3/SYNE3 is at 112 kDa.)

Nesprin3/SYNE3, Polyclonal Antibody (Cat# AAA19982)

Full Name

Anti-Nesprin3/SYNE3 Antibody Picoband

Gene Names

SYNE3; NET53; Nesp3; C14orf49

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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